电针对性发育期雄性大鼠睾酮和精子发生影响的实验观察  

Experimental observation on the effect of electro-acupuncture on the serum testosterone and spermatogenesis of male rats during pubertal development

作　　者：张朝晖[1] 仲远明[1] 崔毓桂[2] 王雪松[2] 居小斌[2] 丁贵鹏[2] 

机构地区：[1]南京医科大学第一附属医院针灸科,江苏南京210029 [2]南京医科大学第一附属医院生殖中心实验室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第4期500-502,506,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省135医学重点人才项目(RC2002076)

摘　　要：目的:观察电针(EA)对不同发育阶段正常大鼠血清睾酮(T)和精子发生的效应。方法:将109只SD雄大鼠按不同年龄段分为幼年段(JG)、青春发育早期段(EPG)、青春发育后期段(LPG)、成年段(AG);每个年龄段随机分为穴位组(EWA)、非穴位组(ENA)、非特异刺激组(IC)和自然对照组(NC)。EWA组选取"百会"、"太溪"、"命门"、"肾俞"穴。3Hz的EA分别刺激EWA组与ENA组大鼠10天,每天20 min。10天后,测定所有大鼠血清T值和精子计数。结果:持续电针后,EPG、LPG与AG中的大鼠血清T值均显著下降(P<0.05),LPG与AG中的大鼠精子数量也明显减少(P<0.05)。结论:持续EA刺激可减少性发育后雄性大鼠血清T的含量和精子数量。Objective：To observe the effect of electro-acupuncture（EA） on serum testosterone（T） and spermatogenesis in normal rests at different developmental stages.Methods：Sprague-Dawley rats were assigned into four groups：juvenile group（JG）,early-puberty group（EPG）,later-puberty group（LPG）and adult group（AG）.Each group was subdivided into four teams：EA at acupoints（EWA）,EA at non-acupoints（ENA）,immobilization control（IC）,normal control（NC）.EA（3 Hz）at EWA or ENA was performed for 20 min per day in different developmental group of rats for 10 days.Sperm number was counted and serum level of T was measured after EA treatment.Results：The results showed that the serum level of T in the EPG,LPG and AG rats were significantly depressed（P 0.05）.Sperm counts in the EPG,LPG and AG groups were also significantly reduced.Conclusion：The results suggested that repeated stimulation with EA is a good option to reduce the serum T and sperm quantity of male rats after the puberty period.

关 键 词：电针 睾酮 精子 

分 类 号：R224[医药卫生—中医基础理论]