两种常用真菌染色法在真菌性角膜炎应用的优缺点比较  

The Advantages and Disadvantages of GMS and PAS Staining in Fungal Keratitis

作　　者：郑健樑[1] 张文忻[1] 林健贤[1] 李永平[1] 丁运刚[1] 姚娟[1] 张平[1] 

机构地区：[1]中山大学中山眼科中心中山大学眼科学国家重点实验室,广州510060

出　　处：《Eye Science》2010年第2期67-69,共3页眼科学报（英文版）

摘　　要：目的:比较高碘酸-无色品红法(PAS)与改良的六胺银染色法(GMS)在真菌性角膜炎诊断应用中的优缺点。方法:回顾性分析该科室自2007年11月至2010年6月诊断为真菌性角膜溃疡的102例标本,统计分析不同染色方法的阳性率,并对染色要点进行分析总结。结果:GMS对真菌性角膜炎的真菌显示率为100%,PAS则为93.14%。结论:GMS对真菌性角膜炎的真菌显示效果优于PAS,且较PAS染色省时、容易操作,不易褪色,不失为真菌染色的最佳选择。但是PAS染色显示真菌的分隔结构较为清晰,可排除色素细胞等的干扰,可以作为真菌性角膜炎鉴别诊断的补充染色。Purpose：To compare the advantages and disadvantages of GMS and PAS Staining in the diagnosis of fungal keratitis. Methods：.Retrospectively analysed the sections of 102 cases of fungal keratitis from November 2007 to June 2010 in our department. Stastically analysed the positive rate of different statining methods. And summarized technical cruxes of GMS and PAS staining. Results： The positive rates of GMS staining and PAS staining for fungal keratitis were 100% and 93.14%, respectively. Conclusions： GMS staining is better than PAS staining for fungal keratitis in displaying fungi. And it takes less time and is easier in operation and makes a more durable colour than PAS staining. But PAS staining is better than GMS staining in displaying fungal septa and eliminating the interference from pigment-containing cells and other cells.

关 键 词：真菌 角膜炎 GMS PAS 

分 类 号：R772.21[医药卫生—眼科]