数字化裂隙灯显微镜测量一维尺寸的可靠性研究  

Study of the Reliability in one Dimensional Size Measurement with Digital Slit Lamp Microscope

作　　者：王涛[1] 戚朝秀[1] 李奇根[1] 董丽洁[1] 杨捷铮[1] 

机构地区：[1]中山大学附属第三医院眼科,广州510630

出　　处：《Eye Science》2010年第2期89-92,共4页眼科学报（英文版）

摘　　要：目的:探讨数字化裂隙灯显微镜作为一种定量分析的工具应用于一维尺寸测量的可靠性。方法:3位观察者采用单盲法,分别在4倍、10倍、16倍、25倍、40倍和4倍、10倍、16倍的物镜放大率下,对游标卡尺上模拟人眼的瞳孔和角膜直径的4.00mm和10.00mm间距,使用国产数字化裂隙灯显微镜进行图像采集和重复测量,对测量的结果的正确度和精密度进行比较。结果:3位测量者(A、B、C)对4.00mm尺寸的图像进行测量,所测结果均数位于3.98～4.06之间。对10.00mm的图像进行测量,所测结果均数位于10.00～10.04之间。4.00mm测量结果显示,除测量者A4倍、测量者B25倍、测量者C16倍和25倍外,测量值与真实值的差异具有统计学意义。10.00mm测量结果显示,除测量者A10倍外,测量值与真实值的差异具有统计学意义。比较同一测量者对同一尺寸在不同的放大倍率的测量结果,除测量者A对10.00mm尺寸的测量无统计学差异外,其余测量结果之间者在不同放大倍率下对相同尺寸测量的结果均具有统计学差异。不同测量者对相同物镜放大倍率下的同一尺寸比较,除在4倍放大率下对4.00mm测量的结果无统计学差异外,其余测量结果之间的差异均具有统计学意义。所有测量结果的变异系数均低于5%;随放大倍率的增加,变异系数有降低的趋势。结论:数字化裂隙灯显微镜是作为一种一维尺寸测量工具,具有良好的精密度,在利用其做定量分析前进行测量的可靠性分析有助于减少系统误差对测量结果分析的干扰。Purpose：To study the reliability in one dimensional size measurement with digital slit lamp microscope as a tool for quantitative analysis. Methods：Three single-blinded observers acquired and repeatedly measured the images of 4.00 mm and 10.00 mm size on the vernier caliper, which simulated the human eye pupil and cornea diameter, with home-made digital slit lamp microscope, respectively, in the objective magnification of 4 times, 10 times, 16 times, 25 times, 40 times and 4 times, 10 times, 16 times. The correctness and precision of measurement were compared. Results：Measurements were conducted on the of 4.00 mm images size by three investigators measurement and all averages were located between 3.98 to 4.06, and 10.00 mm images size 10.00 ～ 10.04. Measurement results of 4.00 mm showed, except A4, B25, C16 and C25, the difference of the measured value and the true value was statistically significant. And measurement results of 10.00 mm showed, except A10, the difference of the measured value and the true value was statistically significant. Comparison of the same size, the same investigators at different magnification measurements, except for investigators A＇s measurements of 10.00 mm dimensions had no significant difference, the others were all statistically significant. Compared the measurements of the same size with different magnification, except measurements of 4.00 mm in 4-fold magnification had no significant difference among the investigators＇, the remaining different results were statistically significant. The coefficient of variation of all measurement results were less than 5%; as magnification increased, the coefficient of variation decreased. Conclusion：The measurement of digital slit lamp microscope in one-dimensional size, with good reliability, should be used analysis reliability before used in quantitative analysis.

关 键 词：裂隙灯 测量 误差 定量分析 

分 类 号：R77[医药卫生—眼科]