Isolation of a Prodigiosin-producing Strain and Analysis of It's Pigments  

一株灵菌红素产生菌的分离及其色素分析(英文)

作　　者：段学辉[1] 傅奇[1] 魏斌[1] 郭炳其[1] 贾奎艳[1] 

机构地区：[1]食品科学与技术国家重点实验室南昌大学,江西南昌330047

出　　处：《Agricultural Science & Technology》2010年第11期25-27,72,共4页农业科学与技术（英文版）

摘　　要：[Objective] The aim was to isolate a prodigiosin producing strain and study its pigment fractions.[Method] Red pigment-producing bacteria was identified by physiological and biochemical characteristics after isolation in plate.By using column chromatography and thin-layer chromatography,pigment fractions were separated and purified from the extractives of the strain after fermentation in flask,and then pigment fractions were analyzed via UV-Vis and LC/MS.[Result] A red pigment-producing Serratia marcescens strain NS-17 sampled from soil of Nanchang was isolated and identified.2 pigment fractions showing similar UV-Vis and LC/MS characters were separated and purified,the characters of fraction 1 were identical to those of prodigiosin,while fraction 2 showed a special UV-Vis absorption spectrum that had not been reported.[Conclusion] A prodigiosin-producing Serratia marcescens strain NS-17 and its 2 pigment fractions were isolated.[目的]从土壤中筛选出灵菌红素产生菌,并对其色素组成进行分析。[方法]利用平板筛选土壤中产红色素细菌,通过生理生化试验进行初步鉴定,摇瓶发酵后提取色素,色素组分经柱色谱与薄层色谱分离纯化后以紫外—可见光吸收光谱(UV-Vis)与液质联用(LC/MS)技术进行分析。[结果]从南昌地区土壤中分离得到1株产红色素的细菌NS-17,生理生化特征与粘质沙雷氏菌(Serratia marcescens)吻合,从发酵后的菌体中分离得到2个具有相似UV-Vis与LC/MS特征的色素组分,组分1分析结果与已报道的灵菌红素一致,组分2具有未见报道的碱性条件下特异性UV-Vis图谱。[结论]分离得到1株产灵菌红素的粘质沙雷氏菌NS-17,并从该菌中分离得到2个色素组分。

关 键 词：Red pigment Serratia marcescens PRODIGIOSIN UV-VIS LC/MS 

分 类 号：Q93[生物学—微生物学]