HPLC法测定菊苣中黄酮类成分的含量  

Determination of Flavonoids in Cichorium intybus L.by HPLC

作　　者：娄猛猛[1] 李国玉[2] 高建波[2] 杨璐[2] 王金辉[2] 

机构地区：[1]石河子大学药学院 [2]石河子大学药学院教育部省部共建新疆特种植物药资源重点实验室,新疆石河子832002

出　　处：《农垦医学》2010年第6期490-493,共4页Journal of Nongken Medicine

基　　金：国家重大新药创制项目(2010ZX09401-304)

摘　　要：目的:建立HPLC法测定菊苣中黄酮类成分含量的方法。方法:色谱柱:Scienhome C18(4.6mm×200mm,5μm),检测波长350nm;柱温30℃;流速1mL/min。结果:木犀草素-7-O-β-D-葡萄糖苷在0.0024～0.0192μg内线性良好,y=2000000x-443.39r,=0.9999,回收率为102.7%,RSD为0.82%;木犀草素在0.01464-0.11712μg内线性良好,y=2000000x-2058.1r,=0.9999,回收率为103.4%,RSD为0.52%。结论:采用该方法进行含量测定准确可靠,重现性好。Objective： To develop a HPLC method for the determination of Flavonoids in Cichorium intybus L.Methods：HPLC was carried out on a Purospher star Scienhome C18（4.6mm×200mm,5μm） at 30℃,at a flow rate of 1.0 mL /min.The detection wavelength was 350 nm.Results：The linearity of luteolin-7-O-β-D-glucoside was found in the range of 0.0024-0.0192μg,r=0.9999,the average recovery was 102.7%,RSD= 0.82%;The linearity of luteolin was found in the range of 0.01464-0.11712μg,r=0.9999,the average recovery was 103.4%,RSD= 0.52%.Conclusion：This method is reliable,accurate and suitable for the determination of Flavonoids in Cichorium intybus L.

关 键 词：HPLC 菊苣 黄酮 

分 类 号：R286[医药卫生—中药学]