CD25^+ Foxp3^+调节性T细胞和Th17细胞在口腔白斑组织中的表达  

Expression of CD25^+ Foxp3^+ Regulatory T Cells and Th17 Cells in Oral Leukoplakia

作　　者：白杨[1] 王文梅[1] 王翔[1] 黄晓峰[1] 蒋文晖[1] 刘雅菁[1] 

机构地区：[1]南京大学口腔医学院.南京市口腔医院口腔黏膜病科,江苏南京210008

出　　处：《口腔医学研究》2011年第3期221-224,228,共5页Journal of Oral Science Research

基　　金：国家自然科学基金项目(编号:18070839);南京市医学科技发展重点项目(编号:zkx10030)

摘　　要：目的:通过观察CD25+Foxp3+和IL-17阳性细胞在口腔黏膜白斑(oral leukoplakia,OLK)和口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织中的表达情况,探讨Treg细胞和Th17细胞在口腔癌发生发展过程中的变化。方法:采用免疫组织化学方法检测CD25+Foxp3+、IL-17阳性细胞在10例单纯增生性白斑、32例异常增生性白斑、13例OSCC和10例正常口腔黏膜中的变化情况。结果:正常口腔黏膜组、单纯增生性白斑组、异常增生性白斑组和OSCC组CD25+Foxp3+Treg细胞数量逐渐增高(P<0.05),依次为(2.5±0.65)、(5.22±1.19)、(31.4±16.8)和(42.8±12.67)个/每高倍镜视野。重度异常增生白斑组织中,CD25+Foxp3+Treg细胞的数量高于轻、中度异常增生白斑组织(P<0.05)。此外,正常口腔黏膜组、单纯增生性白斑组、异常增生性白斑组和OSCC组中表达IL-17的阳性细胞虽有递增趋势,但差异无统计学意义。CD25+Foxp3+Treg细胞和Th17细胞数量改变在口腔白斑和OSCC组织中呈正相关(r=0.318,P<0.05)。结论:Treg细胞的数量在白斑和OSCC组织中增多,Th17细胞协同Treg细胞,在口腔癌发生发展过程中发挥了一定的作用。Objective：To investigate the role of Treg and Th17 during the development and progression of the premalignant lesions.Methods：We compared CD25/Foxp3 and IL-17 expression detected by immunohistochemical staining in 42 oral leukoplakia（with or without dysplasia）,and compared with 10 normal oral mucosa and 13 oral squamous cell carcinoma.Results：There was increasing trend of expression of CD25＋Foxp3＋Treg cells between the cases of normal oral mucosa,oral leukoplakia with hyperplasia epithelium,oral leukoplakia with dysplasia epithelium and OSCC（P0.05）.（They were numerated as 2.5±0.65,5.22±1.19,31.4±16.8 and 42.8±12.67 per high magnification field,respectively）.Further,expression of CD25＋Foxp3＋Treg cells was higher in severe dysplasia OLK than in mild/moderate dysplasia OLK.In addition,there was increasing trend of IL-17 expression which was found between the four groups of normal oral mucosa,oral leukoplakia with hyperplasia epithelium,oral leukoplakia with dysplasia epithelium and OSCC,but no statistical significances.Positive correlation was shown between the density of the subset of co-expression of CD25＋ and Foxp3＋ T cells and expression of IL-17（r =0.318,P0.05）.Conclusion：In assistance with Th17 Cells,Treg cells has played a certain role in the development of oral cancer.

关 键 词：CD25+Foxp3+调节性T细胞 TH17细胞 口腔黏膜白斑 免疫组织化学 双标记染色 

分 类 号：R781.5[医药卫生—口腔医学]