伴颈动脉粥样硬化脑梗患者CD4＋CD28null T细胞CD158j表达和ERK磷酸化水平的关系  

Relationship between CD158j expression and phosphorylated ERK levels in CD4 ＋ CD28noll T cells in cerebral infarction patients with carotid atherosclerosis

作　　者：陈葆国[1] 郑瑞[1] 颜卫华[1] 陈秋月[2] 石卫武[1] 李伯利[1] 

机构地区：[1]浙江省温州医学院附属台州医院中心实验室,临海317000 [2]浙江省温州医学院附属台州医院神经内科,临海317000

出　　处：《中华微生物学和免疫学杂志》2011年第3期269-272,共4页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：浙江省自然科学基金资助项目（Y2080618）

摘　　要：目的 探讨伴颈动脉粥样硬化（CA）脑梗患者CD4＋CD28null T细胞上CD158j的表达百分率和ERK磷酸化（p-ERK）水平的关系及对颈动脉粥样斑块不稳定的影响.方法 采用流式细胞术检测106例伴CA脑梗、33例颈动脉正常脑梗患者和50例健康人外周血CD4＋CD28null T细胞数量,以及CD4＋CD28null T细胞CD158j和穿孔素（perforin）的表达,36例斑块不稳定患者CD4＋T细胞p-ERK水平.ELISA法检测血清IFN-γ水平.B超检查以上人群颈动脉血管内壁粥样硬化情况.结果 脑梗患者CD4＋CD28nullT细胞数量、CD4＋CD28null T细胞CD158j和perforin的表达,以及血清IFN-γ水平,均明显高于健康对照组（P均〈0.01）;CD4＋CD28null T细胞数量、CD4＋CD28null T细胞CD158j和perforin的表达,以及血清IFN-γ水平,在脑梗患者中由高到低依次为颈动脉不稳定斑块组、颈动脉稳定斑块组、颈动脉内-中膜厚度（IMT）增厚组和颈动脉正常组.颈动脉不稳定斑块脑梗患者CD4＋CD28null T细胞CD158j的表达与p-ERK水平呈显著性正相关（P〈0.01）.结论 伴CA脑梗患者CD4＋CD28null T细胞数量明显增多.CD158j可能通过上调p-ERK水平,促进CD4＋CD28null淋巴细胞增殖,产生细胞毒效应和分泌细胞因子,最终导致颈动脉粥样斑块不稳定.Objective To investigate the relationship between CD158j expression and phosphorylated ERK （p-ERK） in CD4＋ CD28null T cells in cerebral infarction （CI） patients- with carotid atherosclerosis and its effects on carotid atherosclerotic plaque stability. Methods Percentage of peripheral CD4＋ CD28null and the expression of CD158j and perform on CD4＋ CD28null cells was analyzed with flow cytometry in 106 CI patients with carotid atherosclerosis, 33 CI patients with normal carotid arteries and in 50 normal controls, respectively; p-ERK expression was assayed with flow cytometry in 36 CI patients with unstable plaque, and serum IFN-γ was detected with ELISA. The intima-media thickness （IMT） of bilateral carotid arteries in all subjects was confirmed by the colour Doppler ultrasonograph imagingResults Percentage of the CD4＋ CD28null T cells, expression of CD158j and perform on CD4＋ CD28null T cells and the serum IFN-γ levels was dramatically higher in CI patients than that in normal controls, respectively （all P 〈0.01）, which was decreased in an order of CI patients with patients with unstable plaque, stable plaque, carotid artery IMT and with normal carotid artery. A strong positive correlation was observed between the CD158j expression and degree of p-ERK in CI patients with unstable plaque （P 〈 0. 01）. Conclusion CD4＋ CD28null T cells were significantly increased in CI patients with carotid atherosclerosis. CD158j might up-regulate p-ERK expression and induce the proliferation of the CD4＋ CD28nullT cells; consequently, higher cytokine production such as IFN-γ produced by CD4＋ CD28null T cells may cause the formation of unstable plaque.

关 键 词：脑梗 颈动脉粥样硬化 CD4+CD28null T细胞 CD158j 磷酸化ERK 

分 类 号：R743.33[医药卫生—神经病学与精神病学]