肾纤维囊为载体培养角膜内皮细胞的实验研究  

Experimental studies on kidney fiber capsule as carriers for cultivating corneal endothelial cells

作　　者：唐光霞[1,2] 陈建苏[1,2] 徐锦堂[1,2] 

机构地区：[1]暨南大学医学院眼科研究所 [2]暨南大学-香港中文大学再生医学教育部重点实验室,广东省广州市510632

出　　处：《眼科新进展》2011年第4期304-307,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30973244);广东省科技计划项目基金资助(编号:2001A302020102;2006B35801003);暨南大学侨办重点学科基金资助(编号:51205004)~~

摘　　要：目的探索以脱细胞的肾纤维囊作为载体培养角膜内皮细胞并研究角膜内皮细胞特性。方法将培养扩增的角膜内皮细胞分别接种到培养板和肾纤维囊上进行体外培养,采用倒置显微镜、免疫荧光、HE染色和扫描电镜的方法进行检测,观察角膜内皮细胞在培养板和肾纤维囊上的生长情况。结果角膜内皮细胞在肾纤维囊上快速贴壁生长并增殖,细胞形态为多角形或六边形,多次传代后细胞仍维持原有的形态和功能,细胞能长期培养。在培养板上培养的角膜内皮细胞贴壁生长和增殖情况稍差。结论角膜内皮细胞在脱细胞的肾纤维囊载体上生长良好,细胞形态结构明显。本研究为角膜内皮细胞的体外培养提供了简单和高效的方法。Objective To investigate the feasibility of kidney fiber capsule as carriers for cultivating corneal endothelial cells and study the characteristics of the cultured cells. Methods Amplified corneal endothelial cells were seeded on culture plates or kidney fiber capsule,and cultured in vitro.Growths of endothelial cells on culture plates and kidney fiber capsule were identified with inverted microscope,immunofluorescence,HE staining and scanning electron microscopy. Results The results indicated that corneal endothelial cells attached firmly on the surface of kidney fiber capsule,and grew quickly.Endothelial cells showed polygon or hexagon morphology.Cells could keep the instinct morphology and function after several passage,and cells could be cultured for a long time.Growth and proliferation of cells on culture plate were poor. Conclusion Corneal endothelial cells on kidney fiber capsule grow well and with obvious cell morphology.This study provides a simple and efficient way for the cells cultured in vitro.

关 键 词：肾纤维囊 角膜内皮细胞 细胞培养 

分 类 号：R772.2[医药卫生—眼科]