NR1、NR2A与PSD-95在生后大鼠海马发育中的表达  

Different expression of NR1,NR2A and PSD-95 in rat hippocampal subregions during postnatal development

作　　者：凌薇[1] 刘伟亚[1] 宋一志[1] 陆涛[1] 常丽荣[1] 武艳[1] 

机构地区：[1]首都医科大学解剖学教研室,北京100069

出　　处：《神经解剖学杂志》2011年第2期147-153,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81071013);北京市自然科学基金(7102011)

摘　　要：目的：探讨N-甲基-D-天冬氨酸受体亚单位1（N-methy1-D-aspartate receptor subunit1,NR1）、N-甲基-D-天冬氨酸受体亚单位2A（N-methy1-D-aspartate receptor subunit2A,NR2A）与突触后密度蛋白-95（Postsynapticdensity protein 95,PSD-95）在Wistar大鼠海马生后发育过程中的表达。方法：应用免疫荧光染色方法检测NR1、NR2A与PSD-95在生后不同时期大鼠海马CA1、CA3区和齿状回（DG）中的表达情况。结果：NR1于生后各期海马CA1、CA3区和DG的表达均增强,P14~P21达高峰期后减弱。NR2A于生后在海马CA1和CA3区的表达略有减弱,P4后增强至高峰期P14,然后轻微减弱,在DG中NR2A的表达生后略有减弱,P4后在增强的趋势中于P7、P14和P28后均有不同程度的减弱,高峰期在P28。PSD-95于生后各期海马CA1、CA3区和DG的表达均增强,P21~P28达高峰期后轻微减弱。结论：NR1、NR2A和PSD-95在CA1、CA3区和DG中的表达具有特异的时空分布模式。Objective：To investigate the expression of N-methy1-D-aspartate receptor subunit 1（NR1）,N-methy1-D-aspartate receptor subunit 2A（NR2A）,and postsynaptic density protein 95（PSD-95） in Wistar rat hippocampus during postnatal development.Methods：Immunofluorescent staining was applied to observe the expression of NR1,NR2A,and PSD-95 in the CA1,CA3,and dentate gyrus（DG） of rat hippocampus during postnatal development.Results：NR1 expression increased with age,reached a plateau at postnatal day 14-21（P14-P21） and then declined.In CA1 and CA3,NR2A expression declined slightly from P0 to P4.After P4,NR2A expression increased with age,reached a plateau at P14 and then declined slightly.In DG,NR2A expression in total increased with age,and reached a plateau at P28,however,at the time points of P0,P7,P14 and P28,it expression decreased gently.PSD-95 expression increased with age,reached a plateau at P21-P28,and then declined slightly.Conclusion：The expression of NR1,NR2A,and PSD-95 has specific temporospatial distribution pattern.The specific temporospatial distribution pattern may be important to the different physiological functions of NMDA receptor during postnatal development.

关 键 词：NR1 NR2A PSD-95 海马 发育 大鼠 

分 类 号：R338[医药卫生—人体生理学]