CIIH预处理对大鼠脑低氧损伤后顶叶皮层c-Fos、Bcl-2表达的影响  

Effects of CIIH preconditioning on the expression of c-Fos and Bcl-2 in parietal cortex after hypoxia-induced injury

作　　者：黄玉哲[1] 钱金泽[2] 王慧娟[1] 钱俊乔[3] 王立轩[1] 张翼[4] 李陈莉[1] 

机构地区：[1]河北医科大学人体解剖与组织胚胎学教研室,石家庄050017 [2]河北医科大学病理学教研室,石家庄050017 [3]河北医科大学临床学院,石家庄050017 [4]河北医科大学生理学教研室,石家庄050017

出　　处：《神经解剖学杂志》2011年第2期179-184,共6页Chinese Journal of Neuroanatomy

基　　金：河北省科技厅课题(042761170)

摘　　要：目的:探讨慢性间歇性低压低氧(CIIH)预处理对大鼠脑低氧损伤后顶叶皮层c-Fos、Bcl-2表达的影响。方法:雄性成年大鼠48只,随机分为4组:正常对照组(CON)、慢性间歇性低压低氧组(CIIH)、急性低氧组(AH)和低氧预处理组(CIIH+AH)。采用甲苯胺蓝染色、免疫组织化学、透射电镜等方法观察大鼠脑顶叶皮层c-Fos、Bcl-2表达的变化。结果:CIIH组未见损伤变性的改变,与CON组相比差异无显著性(P>0.05)。与CON组和CIIH组相比较,AH组可见大鼠脑顶叶皮层神经元出现较为严重的损伤变性的改变,c-Fos、Bcl-2表达显著增加(P<0.01)。与AH组相比,CIIH+AH组神经元损伤变性减轻,Bcl-2表达增高(P<0.05),c-Fos表达降低(P<0.05)。结论:CIIH预处理可能通过上调大鼠脑低氧损伤后Bcl-2表达,下调c-Fos表达,抑制缺氧对神经元凋亡的诱导作用,从而发挥神经保护作用。Objective：To explore the effect of chronic intermittent hypobaric hypoxic（CIIH） preconditioning on the expression of c-Fos and Bcl-2 in hypoxia injured parietal cortex of rats.Methods：Fourty-eight adult healthy male SD rats were randomly divided into control group（CON）,chronic intermittent hypobaric hypoxia group（CIIH）,acute hypoxia group（AH） and hypoxic preconditioning group（CIIH＋AH）.Toluidine blue staining,immunohistochemistry technique and transmission electron microscope were used to monitor the expression of c-Fos and Bcl-2 in parietal cortex.Results：No obvious neural denaturation in parietal cortex was found in CIIH group.Between the two groups of CON and CIIH,there was no statistical difference（P〉0.05）.Compared with CON group and CIIH group,neural serious denaturation in parietal cortex was found in AH group.The expression of c-Fos and Bcl-2 in AH group was significantly increased（P〈0.01）.Compared with AH group,the neurons in CIIH＋AH group were more slightly injured,the expression of Bcl-2 was increased（P〈0.05）,while the expression of c-Fos was reduced（P〈0.05）.Conclusion：CIIH preconditioning may inhibit hypoxia-induced apoptosis by down-regulation of c-Fos expression and up-regulation of Bcl-2 expression,hereby protecting the brain

关 键 词：慢性间歇性低压低氧 低氧损伤 BCL-2 C-FOS 凋亡 大鼠 

分 类 号：R363[医药卫生—病理学]