茚三酮比色法和甲醛滴定法测定水解胶原的比较  

Comparison of Ninhydrin Colorimetry and Formaldehyde Titration in Determination of Collagen Hydrolysate

作　　者：崔敏[1] 傅婕[1] 迟原龙[1] 廖学品[1] 石碧[1] 

机构地区：[1]四川大学制革清洁技术国家工程实验室,四川成都61006

出　　处：《中国皮革》2011年第7期1-4,共4页China Leather

基　　金：国家自然科学基金(20976111)资助项目

摘　　要：研究了茚三酮比色法和甲醛滴定法测定氨基酸含量的线性检测范围、检测精密度和准确度。结果表明:茚三酮比色法的线性检测范围为0.5～6mg/L,最低可检测浓度为0.5mg/L,测定浓度范围广;甲醛滴定法最低可检测浓度为2g/L,适合测定高浓度样品。2种方法的相对标准偏差(RSD)<2.5%,回收率在98%～103%之间,表明这2种检测方法均具有较好的重复性和较高的准确度。这2种方法用于胶原水解产物测定时,茚三酮比色法的测定值总是低于甲醛滴定法的测定值,这是由于胶原水解产物的脯氨酸和羟脯氨酸含量较高,而这2种氨基酸在茚三酮比色法测定波长568nm处,无最大吸收峰。The linear range,precision and accuracy of ninhydrin colorimetry and formaldehyde titration in determining amino acid content were investigated,respectively. The ninhydrin colorimetry with a lowest detection limit of 0. 5mg/L presents a broad detecting range of amino acid concentration. The lowest detection limit for formaldehyde titration is 2. 0g/L,and therefore,it is a method suitable for determining high concentration samples. The relative standard deviations （ RSD） of the two methods are all less 2. 5% and their recovery coefficient of determination is in the range of 98% to 103% ,which indicates that both of the methods have satisfactory precision and accuracy. As for determination of amino acid content in collagen hydrolysate,the value tested by ninhydrin colorimetry is always lower than that obtained by formaldehyde titration. This is should be due to the fact that the collagen hydrolysate contains a relatively high content of proline and hydroxyproline,and these two amino acids have no maximum absorption at 568nm where ninhydrin colorimetry works.

关 键 词：茚三酮比色法 甲醛滴定法 胶原 水解胶原 线性检测范围 精密度 准确度 

分 类 号：TS54[轻工技术与工程—皮革化学与工程]