酶联免疫吸附法测定血小板反应素1及其对前列腺癌诊断价值的研究  

Study on enzyme-linked immunosorbent assay for detecting thrombospondin-1 and its diagnostic value for prostatic carcinoma

作　　者：张耀光[1] 王建业[1] 齐若梅[3] 张力青[1] 万奔[1] 魏东[1] 朱生才[1] 何美懿[2] 陈仁涉[3] 曾品玲 

机构地区：[1]卫生部北京医院泌尿外科,100730 [2]卫生部北京医院检验科,100730 [3]卫生部北京老年医学研究所免疫研究室 [4]北京首都国际机场医院检验科

出　　处：《中华老年医学杂志》2011年第4期305-309,共5页Chinese Journal of Geriatrics

摘　　要：目的 采用酶联免疫吸附法（ELISA）测定血小板反应素-1（TSP-1）,并分析TSP-1对前列腺癌的诊断价值.方法 用ELISA斑点法测定TSP-1,研制了3种具有分离特异抗原功能的试剂,研发了一项具有纯化蛋白能力的技术,解决非特异蛋白的交叉反应问题,建立测定TSP-1的ELISA常规方法.并用该方法测定14例良性前列腺增生和18例前列腺癌患者血清标本的TSP-1.结果 前列腺癌组TSP-1值平均（73.77±12.72）%,前列腺增生组为（121.86±19.47）,差异有统计学意义（t=8.44,P＜0.01）.TSP-1和前列腺特异性抗原（PSA）对前列腺癌诊断的敏感性和特异性分别为92.9%、88.9%和85.7%、66.7%,差异有统计学意义（均P＜0.01）.TSP-1的受试者工作特征曲线（ROC）曲线下面积为0.9663,PSA的ROC曲线下面积为0.7421,差异有统计学意义（P＜0.05）.结论 应用ELISA方法测定TSP-1现实可行,TSP-1是一种理想的前列腺癌诊断指标,可能比PSA更能够准确地鉴别良性和恶性前列腺疾病.Objective To use enzyme-linked immunosorbent assay （ELISA） for measuring thrombospondin-1 （TSP-1）,and to analyze its diagnostic value for prostatic carcinoma.Methods The possible difficulties and the way to solve the difficulties with ELISA spot were explored first.Three agents which could segregate idio-antigen and one technique which could depurate proteinum were designed and developed.The non-idio- proteinum cross reaction problems were solved and the routine method to measure TSP-1 with ELISA was set up successfully.The serum TSP-1 was measured in 14 patients with benign prostatic hyperplasia （BPH） and 18 patients with prostatic carcinoma.Results The TSP-1 values were （73.77±12.72）% and （121.86±-19.47）% in prostatic carcinoma group and benign prostatic hyperplasia group,respectively （t= 8.44,P〈0.01）.The diagnostic sensitivity and specificity of TSP-1 and prostate specific antigen （PSA） for prostatic cancer were 92.7%,88.9% and 85.7%,66.7%,respectively （P〈0.01）.The area under the receiver operating characteristic curve （ROC） of TSP-1 and PSA were 0.9663 and 0.7421 （P〈0.05）.Conclusions The determination of TSP-1 with ELISA is feasible.TSP-1 is an ideal diagnostic parameter for prostatic carcinoma and it may distinguish BPH from malignant prostatic disease more exactly than PSA.

关 键 词：前列腺增生 前列腺肿瘤 血小板 反应素类 

分 类 号：R737.25[医药卫生—肿瘤]