大鼠实验性牙移动中配基门控阳离子通道P2X_3受体在牙髓中的表达变化  

Expression of ligand-gated cation channels P2X_3 receptor in rat pulp during experimental tooth movement

作　　者：卢云[1,2] 杨秩[3] 华小川[4] 常新[1] 贵林[1] 王晟[2] 赖文莉[2] 

机构地区：[1]大连医科大学口腔医学院正畸教研室,大连116044 [2]四川大学华西口腔医院正畸科,成都610041 [3]上海交通大学医学院附属第九人民医院正颌正畸联合治疗中心,上海200011 [4]云南省第二人民医院口腔正畸科,昆明650021

出　　处：《华西口腔医学杂志》2011年第2期183-186,共4页West China Journal of Stomatology

摘　　要：目的探讨大鼠实验性牙移动过程中配基门控阳离子通道P2X3受体在牙髓中表达变化,初步了解P2X3受体在正畸牙移动过程中的可能作用。方法将54只雄性SD大鼠(体重200～250 g)随机分为空白组(5只)、对照组(14只)和实验组(35只)。选择左侧上颌第一磨牙作为观察对象,获取不同时间点牙髓组织切片,进行免疫组织化学研究。结果正畸牙移动加力后,牙髓中P2X3受体免疫阳性表达增加,并呈现一定的时间规律:即受力后1/6 d逐渐增加,3 d达到高峰,7 d后下降至加力前水平。结论正畸牙移动疼痛信息传递过程中P2X3受体呈现有规律变化,推测P2X3受体与牙移动伤害表达密切相关,但其作用机制还有待进一步研究。Objective To investigate ligand-gated cation channels P2X3 receptors changes in rat pulp during experimental tooth movement（ETM）,and preliminarily find their possible effect during ETM.Methods 54 male SD rats（200-250 g） were selected and randomly divided into blank group（5 rats）,control group（14 rats） and experimental group（35 rats）.The left maxillary first molar was selected as observation object,the pulp tissue biopsies was taken at different time points to carry out immunohistochemical study.Results Predominant up regulation of P2X3 receptors immunoactivity was found in pulp from 1/6 d to 7 d after experimental tooth movement.It started to significantly in-crease at 1/6 d,peaked at 3 d,and then decreased continuously until 7 d as compared with the beginning.Conclusion P2X3 receptors have a rhythm change in rat pulp as a result of ETM,speculated that P2X3 receptors is closely related to the tooth movement injury,but the mechanism of action need further researches.

关 键 词：实验性牙移动 牙髓 疼痛 P2X3受体 

分 类 号：R783.5[医药卫生—口腔医学]