蔗糖调节拟南芥花青素的生物合成  

Anthocyanin Biosynthesis Regulated by Sucrose in Arabidopsis thaliana Seedling

作　　者：杨少华[1] 王丽[1] 穆春[1] 王翔[1] 何静辉[1] 赵静尧[1] 王林嵩[1] 

机构地区：[1]河南师范大学生命科学学院,新乡453007

出　　处：《中国生物化学与分子生物学报》2011年第4期364-369,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助项目(No.30570135);河南省生物化学与分子生物学重点学科;国家大学生创新性实验计划资助项目(No.101047603)~~

摘　　要：为了探讨糖在花青素合成过程中的调节作用,采用蔗糖和其代谢糖(葡萄糖和果糖)组合处理拟南芥幼苗.实验结果表明,60 mmol/L蔗糖处理显著提高拟南芥幼苗的花青素、还原糖含量,并上调花青素合成相关基因(CHS,FLS-1,DFR,LDOX,BANYULS)的转录,对叶绿素含量和UGT78D2基因的转录无影响;20 mmol/L葡萄糖+20 mmol/L果糖处理,对花青素、叶绿素和还原糖的含量无影响,对花青素合成相关基因转录影响不一;20 mmol/L蔗糖+20 mmol/L葡萄糖+20mmol/L果糖处理后,花青素和还原糖含量介于前两个处理之间,也上调花青素合成相关基因的转录;但和蔗糖处理组相比,上调UGT78D2基因转录,下调FLS-1基因转录.在不同处理组之间,花青素含量变化和还原糖含量变化趋势相同,有可能糖在调节花青素合成的同时也调节还原糖含量.因此,蔗糖既可以通过蔗糖特异信号途径,也可以和其代谢糖通过其他途径共同调节拟南芥花青素的生物合成.In order to investigate the sugar regulation of anthocyanin biosynthesis,the combined effects of sucrose and its metabolic product of glucose and fructose were studied in Arabidopsis thaliana seedling.The results indicated that when cultured with 60 mmol/L sucrose,the contents of anthocyanin and reductive sugars were significantly increased,and the transcription genes in anthocyanin biosynthesis were upregulated,such as chalcone synthase（CHS）,flavonol synthase-1（FLS-1）,dihydroflavonol reductase（DFR）,leucoanthocyanidin dioxygenase（LDOX）,anthocyanidin reductase（BANYULS）.The chlorophyll content and UDP-Glc： flavonoid 3-O-glucosyltransferase（UGT78D2） expression remained unchanged.When grown at 1∶ 1 mixture of 20 mmol/L glucose ＋ 20 mmol/L fructose,no changes of anthocyanin,chlorophyll and reductive sugars were observed,whereas the expression of anthocyanin biosynthetic genes varied.In case of the treatment with 1：1：1 mixture of 20 mmol/L sucrose ＋ 20 mmol/L glucose ＋ 20 mmol/L fructose,amount of anthocyanin and reductive sugars and the upregulation of anthocyanin biosynthetic enzymes were in between of the other two treatments.Compared with the sucrose alone treatment,the UGT78D2 expression increased.The FLS-1 transcription was down-regulated in three sugar mixture treatment.In three treatments,the changes of anthocyanin correlated with the reductive sugars,implying that the sugar content might regulate the anthocyanin biosynthesis in Arabidopsis thaliana seedling,and perhaps involved either the sucrose-specific sensing and signaling pathway of sugar metabolism.

关 键 词：蔗糖 调节 拟南芥 花青素 还原糖 

分 类 号：Q591.9[生物学—生物化学]