直肠癌组织中低氧诱导因子1α的表达及与微淋巴管密度和淋巴结微转移的关系  

Association of hypoxia-inducible factor 1α expression with microlymphatic vessel density and lymph node micrometastasis in rectal cancer

作　　者：常家聪[1] 朱广玉[1] 杨锦雷[1] 张红[2] 王道斌[3] 

机构地区：[1]安徽医科大学第一附属医院普通外科二病区,合肥230032 [2]安徽医科大学第一附属医院普通外科二病区病理科,合肥230032 [3]安徽医科大学第一附属医院普通外科二病区中心实验室,合肥230032

出　　处：《中华胃肠外科杂志》2011年第4期284-287,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：安徽省卫生厅医学科研资助项目（09A034）

摘　　要：目的 探索低氧诱导因子1α（HIF-1α）在直肠癌组织中的表达情况及其与直肠癌微淋巴管密度（MLVD）和淋巴结微转移的关系.方法 收集安徽医科大学第一附属医院2000-2003年间手术切除并经病理证实的中低位直肠癌标本40例,并取相应癌旁组织作为对照.用免疫组织化学方法测定组织中HIF-1α表达、D2-40表达（计算MLVD值）及CK20表达（判断淋巴结微转移情况）.结果 40例直肠癌与癌旁组织中HIF-1α相对表达水平分别为77 386±14 911和33 092±5877,MLvD值分别为7.3±0.7和0.3±0.2,差异均有统计学意义（P＜0.01）;且两者之间呈正相关（r=0.781,P＜0.01）.淋巴结微转移阳性率为32.3%（10/31）,且淋巴结微转移阳性者HIF-1α表达和MLVD值均显著高于淋巴结微转移阴性者（P＜0.05）.结论 HIF-1α和MLVD在直肠癌的发生、发展过程中具有重要作用,并有可能促进直肠癌淋巴结微转移.Objective To explore the association of the expression of hypoxia-inducible factor 1α （HIF-1α） with microlymphatic vessel density （MLVD） and lymph node micro-metastasis in rectal cancer.Methods The experimental group consisted of 40 middle-low rectal cancer specimens pathologically confirmed at the First Affiliated Hospital of Anhui Medical University between 2000 and 2003.Forty samples of normal tissues taken from the corresponding area around the cancer were used as the control group. Immunohistochemistry was used to detect HIF-1α expression and MLVD in both the tumor tissues and the adjacent normal tissues. Lymph node micrometastasis was ascertained using immunohistochemical staining with CK20. Results In rectal cancer tissues, the HIF-lα expression was 77 386±14 911 and MLVD was 7.3±0.7, significantly higher than those in normal adjacent tissues（33 092±5877 and 0.3±0.2, both P＜0.01）. The HIF-1α expression was positively correlated with MLVD in rectal cancer （r=0.781, P＜0.01）. Thirty-one patients had no lymph nodes metastasis and 10 had micrometastasis. The HIF-1α expression and MLVD in specimens with lymph node micrometastasis was significantly higher than that in those without lymph node micrometastasis（P＜0.05）. Conclusion HIF-1α and MLVD play important roles in the development of rectal cancer, which may promote lymphatic micrometastasis in rectal cancer.

关 键 词：直肠肿瘤 低氧诱导因子1Α 微淋巴管密度 淋巴结微转移 

分 类 号：R735.37[医药卫生—肿瘤]