AnnexinⅠ蛋白在胰腺癌中的表达及其临床意义  

Expression of AnnexinⅠ in pancreatic cancer and its clinical significance

作　　者：付海峰[1] 贺德华[1] 许安荣[1] 张华[1] 李桂中[1] 黄凯中 

机构地区：[1]湖北省东风汽车公司茅箭医院普通外科,湖北十堰442012 [2]湖北省武汉市协和医院胰腺外科中心,湖北武汉430022

出　　处：《中国普通外科杂志》2011年第3期261-264,共4页China Journal of General Surgery

摘　　要：目的探讨AnnexinⅠ蛋白在胰腺癌组织中的表达其临床意义。方法采用免疫组织化学及免疫印迹方法检测40例胰腺癌组织及其相应的癌旁组织、20例正常胰腺组织中AnnexinⅠ蛋白的表达,并分析胰腺癌组织中AnnexinⅠ蛋白的表达与临床病理特征尤其是病理分级、临床分期及转移的关系。结果胰腺癌AnnexinⅠ蛋白阳性表达率为72.5%,癌旁组织为37.5%,正常胰腺组织为20%,三者呈递减趋势,各组之间差异有统计学意义(P<0.05)。胰腺癌AnnexinⅠ蛋白的表达与肿瘤分化程度、TNM分期、淋巴结转移有关。Western blot检测结果与免疫检测方法结果一致。结论 AnnexinⅠ在胰腺癌组织中高表达,且其表达与肿瘤分级有关,提示AnnexinⅠ可能通过免疫机制参与胰腺癌的发生、发展过程。Objective To explore the expression of AnnexinⅠ in pancreatic cancer and its clinical significance. Methods Immunohistochemical method and the Western blot method were used to examine the expression of AnnexinⅠ in pancreatic cancer tissues,tumoral adjacent tissues,and normal pancreatic tissues obtained during operation from 40 pancreatic carcinoma patients and 20 patients with normal pancreas.The relationships with pathological grade,clinical stage and metastasis of pancreatic cancer were statistically analyzed. Results The percentage of positive cells that expressed AnnexinⅠ protein in human pancreatic tissues,tumor adjacent tissues and normal tissues was 72.5%,37.5% and 20.0%,respectively,with the protein expression level of AnnexinⅠ showing gradual decline from cancer to normal tissue（P0.05）.The highly expressed AnnexinⅠ correlated significantly with the degree of tumor differentiation,advanced TNM stage and lymph node metastasis.Similar results were obtained by Western blot test. Conclusions AnnexinⅠ has high expression in pancreatic cancer tissues.AnnexinⅠ expression has a close relationship with pathological grades of pancreatic cancer,suggesting that AnnexinⅠ might play an important role in the development and progression of pancreatic cancer through immunologic mechanism.

关 键 词：胰腺肿瘤/病理学 AnnexinⅠ蛋白 免疫组织化学 免疫印迹 

分 类 号：R735.9[医药卫生—肿瘤]