口腔鳞癌组织中Galectin-1表达及其临床意义  

Galectin-1 expression in oral squamous cell carcinoma ant it's clinical significance

作　　者：魏魁杰[1] 潘红芽[2] 杨筱[2] 韩伟[2] 钟来平[2] 张志愿[2] 

机构地区：[1]湖州师范学院医学院口腔系,313000 [2]上海交通大学医学院附属第九人民医院口腔颌面外科

出　　处：《实用口腔医学杂志》2011年第2期217-221,共5页Journal of Practical Stomatology

基　　金：浙江省自然科学基金资助项目(编号:Y2091171)

摘　　要：目的:探讨口腔鳞癌组织中半乳糖凝集素1(Galectin-1)基因与蛋白质的表达水平及其临床意义。方法:分别采用荧光实时定量RT-PCR和免疫组织化学方法,检测Galectin-1在30例口腔鳞癌患者的癌组织及癌旁组织中mRNA和蛋白质表达水平,其表达水平与临床病理指标之间的关系采用SPSS10.0软件包进行统计分析。结果:口腔鳞癌组织中Galectin-1 mRNA和蛋白质表达水平均显著高于癌旁组织(P<0.01)。Galectin-1的表达水平与烟酒嗜好、肿瘤大小、淋巴结转移、临床分期、病理分化程度无显著相关性。结论:在口腔鳞癌组织中Galectin-1 mRNA和蛋白质表达上升,可能与肿瘤的发生、发展有关。Objective： To investigate the gene transcript level and protein expression of Galectin-1 in oral squamous cell carcinoma（OSCC） and their clinical significance.Methods： The mRNA level and protein expression of Galectin-1 in OSCC of 30 cases and paracancerous tissue were detected by fluorescent quantitive real-time RT-PCR and immunohistochemistry technique respectively.The relationship between the Galectin-1 expression level and the clinicopathological parameters was analyzed by SPSS10.0 software package.Results： The Galectin-1 mRNA transcript level and protein expression in cancerous tissue increased significantly compared with that in the para-cancerous tissue（P0.01）.No significant correlation was found between the Galectin-1 expression and clinical status（P0.05）,such as smoking,drinking,T stage,N stage,clinical stage and pathological differential degree.Conclusion： Up regulation of mRNA level and protein expression of Galectin-1 may be related to the tumorigenesis and development of OSCC.

关 键 词：口腔鳞状细胞癌 半乳糖凝集素1 基因表达 实时定量RT-PCR 免疫组织化学 

分 类 号：R739.8[医药卫生—肿瘤]