排龈时间与药物对牙龈成纤维细胞的毒性比较研究  

Toxicity of different retraction agents and exposure time on human gingival fibroblasts in vitro

作　　者：张晓明[1] 惠敏[2] 刘健 

机构地区：[1]滨州医学院附属医院口腔修复科,256603 [2]滨州医学院口腔学院 [3]日照市中医院口腔正畸科

出　　处：《实用口腔医学杂志》2011年第2期248-251,共4页Journal of Practical Stomatology

基　　金：滨州医学院科技计划(编号:BY2007KJ47)

摘　　要：目的:研究不同排龈药物与不同排龈时间对人牙龈成纤维细胞(HGF)毒性作用的影响,指导临床选择最佳排龈药物和排龈时间。方法:MTT比色测定细胞活性,透射电镜观察直接损伤后细胞超微结构的变化,排龈药物pH值测定。结果:0.1%盐酸肾上腺素的细胞毒性作用最小,排龈时间5 min细胞毒性小于10 min,硫酸铁的细胞毒性最大。结论:健康患者可采用0.1%盐酸肾上腺素进行排龈;伴有心血管系统疾病的患者,可采用20%硫酸铝替代0.1%盐酸肾上腺素;临床上使用硫酸铁排龈时应适当降低其浓度。Objective： To choose the best retraction agent and exposure time for the clinic by investigating cytotoxic effects of three chemical retraction agents on human gingival fibroblasts（HGF） in vitro.Methods： The proliferation inhibit was observed by MTT assay,the ultra structure of the cells was observed under transmission electron microscope.Three chemical retraction agents of pH were determinated.Results： 0.1%HCl-epinephrine was the least toxic gingival retraction agent,and cytotoxic effect of 5min treatment was less than 10 min.15.5% Fe2（SO4）3 was found to have the strongest cytotoxic effect on the cells.Conclusion： 0.1% HCl-epinephrine is suggested for patients without cardiovascular disease.For patient with cardiovascular disease,the better substitute is 20% Al2（SO4）3.15.5% Fe2（SO4）3 can not be used in patients.

关 键 词：排龈药物 细胞毒性 人牙龈成纤维细胞 

分 类 号：R781.05[医药卫生—口腔医学]