环境空气中尼古丁的气相色谱-质谱测定法  

Determination of Environmental Air Nicotine by Gas Chromatograph-Mass Spectrometry

作　　者：董小艳[1] 王秦[1] 徐东群[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《环境与健康杂志》2011年第3期253-255,共3页Journal of Environment and Health

基　　金：国家科技部支撑计划课题(2008BAI56B07)

摘　　要：目的采用被动式采样器采样,气相色谱-质谱法测定环境空气中尼古丁。方法环境空气中的尼古丁用经过NaHSO4处理过的玻璃纤维滤膜采集,滤膜经1.5 mol/L NaOH洗脱和正庚烷萃取后经气相色谱分离,采用质谱选择离子扫描方式采集数据,保留时间和特征离子定性,并以喹啉作为内标物进行定量。结果该方法的检出限为0.01μg/ml,定量下限0.034μg/ml,按采样体积312 L计,线性范围为0.22～16μg/m3。低、中、高3个浓度的标准溶液6次测定的RSD分别为8.4%,6.0%和4.9%;3个浓度的加标回收率范围分别为82.8%～97.4%,94.0%～109.8%和85.4%～109.8%。结论本方法采样方便,测定灵敏、准确,可以在场所监测或人群个体暴露环境烟草烟雾(ETS)监测中大规模使用,为客观评价ETS暴露提供技术手段。Objective To establish a method for the determination of environmental air nicotine by passive diffusion sampling and chromatograph-mass spectrometry analysis. Methods The environmental air nicotine was collected onto the filter treated with sodium bisulfate by diffusing first, and then desorbed and extracted into the solution of 1.5 mol/L sodium hydroxide and heptane from the filter, the nicotine in the heptane was analyzed by gas chromatograph-mass spectrometry with SIM （selected ion monitoring）, quinoline was used as internal standard to quantitative analysis. Results The limit of detection was 0.01 μg/ml and the limit of quantification was 0.034 μg/ml, the linear range of the method was 0.22-16 μg/m3 with 312 L sampling volume, the relative standard deviations of three levels concentrations were 8.4%, 6.0% and 4.9% respectively, and the recovery range of three levels concentrations were 82.8%-97.4% ,94.0%-109.8% and 85.4%-109.8% respectively. Conclusion The method is convenient with the passive sampling, sensitive and accurate with gas chromatograph-mass spectrometry, it can be used in field study for environmental monitoring and personal exposure, and it can provide technical support to evaluate environmental tobacco smoke（ETS） exposure.

关 键 词：气相色谱-质谱 尼古丁 被动式采样 

分 类 号：O657.7[理学—分析化学]