Kiss-1和环氧化酶-2在原发性肝癌中的表达及意义  

Expression level and significance of Kiss - 1 and Cox - 2 in primary hepatocellular carcinoma

作　　者：任连伟[1] 刘超[1] 庞志刚[1] 王广田[1] 尚阎[1] 王彬[1] 

机构地区：[1]郑州大学第二附属医院普外科,450014

出　　处：《中国实用医刊》2011年第10期37-39,共3页Chinese Journal of Practical Medicine

摘　　要：目的研究环氧化酶-2（Cox-2）RNA和Kiss-1RNA在原发性肝细胞肝癌中的表达及临床意义。方法采用RT—PCR技术检测Cox-2RNA和Kiss-1RNA在正常肝组织（10例）、癌旁肝硬化组织（30例）和肝癌组织（39例）的表达情况，并比较它们在不同组织中表达的差异及临床意义。结果Cox-2RNA在正常的肝组织中不表达，在肝硬化组和肝癌组中高表达，但两者比较差异无统计学意义（P〉0．05），但都与正常组比较差异有统计学意义（P〈0．05）。Cox-2RNA的表达与肝癌的大小、静脉浸润、TNM分期、Edmenson分级等无关（P〉0．05）。Kiss-1RNA在正常肝组织．肝硬化和肝癌组织中均有表达，正常组和肝硬化组比较差异无统计学意义（P〉0．05），但都与肝癌组比较差异有统计学意义（P〈0．05）。Kiss-1RNA的表达与TNM分期和Edmenson分级等密切相关（P〈0．05），与肿瘤的大小、静脉浸润等无关（P〉0．05）。结论Cox-2RNA在肝癌中表达明显升高，实验证明它可能与肿瘤的浸润、转移等无关；Kiss-1RNA的表达在肝癌中抑制显著，实验证明它可能与肿瘤的浸润、转移等有密切相关。同时Cox-2RNA和Kiss-1RNA的表达呈显著负相关（P〈0．05）。Objective To investigate the expression and clinical significance of Kiss - 1 RNA and Cox- 2 RNA in primary hepatocellular carcinoma （HCC）. Methods Expression of Kiss - 1 RNA and Cox - 2 RNA in normal liver tissues （ 10 cases）, liver cirrhosis tissues （30 cases） and HCC tissues （39 cases） were detected by RT- PCR,to find the difference in their expression level and the clinical pathology parameters. Results There were no expression of Cox- 2 RNA in normal group and high expression in cirrhosis group and HCC group which each other have little difference and no significance （ P 〉 0.05 ）, but have significantly higher expression compared to normal group （ P 〈0.05）. The Cox -2 RNA expression has no relation with tumor size, vein invasion, TNM stage and Edmenson grade （ P 〉 0.05）. Kiss - 1 RNA ex- pression had a high expression in normal tissues, cirrhosis tissues and HCC tissues, Kiss- 1 RNA expression in HCC tissues was significantly lower than that in normal tissues and cirrhosis tissues（ P 〈0. 05 ） ,but there were no obviously different between normal tissues group and cirrhosis tissues group（ P 〉0. 05 ）. The expression of Kiss -1RNA in HCC tissues was reduced and had significant relation with TNM stage and Edmenson grade of tumor （ P 〈 0.05 ）, but was not related to tumor size and vein invasion （P 〉 0.05 ）. Conclusions The expression of Cox -2 RNA is very high in HCC tissues maybe have no relation with in vasion and metastasis;Kiss- 1 RNA expression is obvious suppressed in HCC tissues,the reduced kiss- 1 RNA expression maybe helpful to the invasion and metastasis of tumor. A close negative correlation between the both genes was showed （ P 〈 0.05）.

关 键 词：原发性肝癌 KISS-1 RNA COX-2 RNA RT—PCR表达水平 相关性 

分 类 号：R735.7[医药卫生—肿瘤]