胶质母细胞瘤中CD133阳性细胞的分布与组织微血管增殖的相关性  

Correlation between the distribution of CD133-positive cells and the proliferation of microvessels in glioblastoma multiforme

作　　者：王华民[1] 杨学军[1] 董雪涛[1] 李瑜[1] 王维[1] 明浩朗[1] 张斌[1] 于圣平[1] 

机构地区：[1]天津市神经病学研究所神经肿瘤实验室,天津医科大学总医院神经外科300052

出　　处：《中华医学杂志》2011年第11期781-785,共5页National Medical Journal of China

基　　金：国家自然科学基金（30772228）

摘　　要：目的 观察胶质瘤干细胞标记物CD133和血管内皮细胞标记物CD34在胶质母细胞瘤及瘤周不同部位的表达情况,探讨胶质瘤干细胞在恶性胶质瘤中的侵袭迁移规律.方法 收集52例胶质母细胞瘤病人的组织标本,每例标本分别取肿瘤中心组织、肿瘤和水肿交界区组织及肿瘤周围水肿区组织3部分.采用免疫组织化学染色和Western印迹法检测CD133在各组标本中的表达情况,采用免疫组织化学染色法检测CD34-微血管在组织标本中的分布,并分析两者的相关性.结果 CD133在对照组组织中不表达,而在肿瘤和水肿交界区、肿瘤中心区,瘤周水肿区组织中免疫组化染色表达分值分别为7.3±1.4、5.2±1.1、2.7±1.0,光学显微镜下可见,在交界区CD133＋细胞分布密集,往往形成以微血管为中心的假菊型团样结构,在中心区CD133＋细胞常在微血管附近簇状分布,在水肿区CD133＋细胞以散在分布为主;Western印迹检测吸光度值分别为0.79±0.03、0.38±0.01、0.20±0.04;各区域间的差异有统计学意义（P＜0.05）.在高倍视野下,肿瘤和水肿交界区、肿瘤中心区、肿瘤周围水肿区、对照组组织中CD34-微血管密度（个/视野）分别是31.3±4.0、21.8±2.6、15.3±2.4、4.7±1.5;各组之间的差异有统计学意义（P＜0.05）.CD133的表达水平和组织微血管的分布呈正相关（r=0.948,P＜0.05）.结论 胶质瘤干细胞向微血管生成丰富的交界区富集,认为他和组织微血管之间可能存在密切的＂营养＂联系;胶质瘤干细胞在胶质母细胞瘤中的分布特征可能与其侵袭迁移有关.Objective To investigate the expression of CD133 and CD34 in different parts of glioblastoma and its margin and explore the invasive path of glioma stem cells within the tumor and surrounding tissue.Methods The surgical specimens were collected from the core of mass,junctional zones between tumor and peritumoral edematous areas and edematous areas in 52 patients with glioblastoma.Immunohistochemical cell staining and Western blot were employed to evaluate the expression of CD133 in different specimens while immunohistochemistry was used to detect the CD34-microvesel postforming.A correlation analysis was performed between them.Results The expression of CD133 was not detected in the control groups while the scores were 7.3 ± 1.4,5.2 ± 1.1,2.7 ± 1.0 in junctional zones,tumor cores and edematous areas with immunohistochemistry and 0.79 ± 0.03,0.38 ± 0.01,0.20 ± 0.04with Western blot respectively.There were significant differences between different groups （P ＜ 0.05）.Under light microscope,the CD133-positive cells frequently forming perivascular pseudorosettes were dense in junctional zones and mostly clustered near the microvessels in tumor cores and scattered in edematous areas.At a high magnification （200 ×）,the CD34-MVD（/HP） values were 31.3 ±4.0,21.8 ± 2.6,15.3 ±2.4,4.7 ± 1.5 respectively in junctional zones,tumor cores,edematous areas and control tissues.Significant differences were also found in these groups（P ＜ 0.05）.The expression level of CD133-positive cell was positively correlated with the distribution of CD34-microvessels （r =0.948,P ＜ 0.05）.Conclusion Glioma stem cells tend to assemble in the junctional zones where the microvessels are enriched.There is probably an intimate nourishing relationship with the microvessels.The distribution of glioma stem cells may be related with the invasiveness within glioblastoma.

关 键 词：胶质母细胞瘤 胶质瘤干细胞 侵袭 微血管密度 

分 类 号：R739.4[医药卫生—肿瘤]