牛蒡子苷元对肝癌侵袭转移的影响  

Treatment with arctigenin inhibits the metastasis and invasion of human hepatocellular carcinoma SMMC-7721 cells

作　　者：王兵[1] 郑国灿[1] 

机构地区：[1]东南大学医学院附属中大医院普外科,江苏省南京市210009

出　　处：《世界华人消化杂志》2011年第7期723-727,共5页World Chinese Journal of Digestology

基　　金：东南大学国家自然基金预研基金资助项目;No. XJ0650261~~

摘　　要：目的:研究牛蒡子苷元(arctigenin,ARG)对肿瘤黏附、侵袭、转移的影响.方法:体外实验分别采用MTT法、Transwell法检测ARG对SMMC-7721细胞黏附、侵袭和转移的影响;体内实验采用裸鼠肺转移瘤模型,检测ARG对SMMC-7721细胞肺转移的影响.结果:与空白对照组相比,ARG作用后SMMC-7721细胞黏附率、侵袭率和转移率显著下降,其平均黏附抑制率、转移抑制率和侵袭抑制率分别为43.08%、55.19%和58.21%.随着ARG浓度的增加,作用时间的延长,ARG对SMMC-7721细胞及细胞黏附的抑制作用显著增强(72h:0.260±0.014vs0.999±0.066,P<0.05;4h:0.558±0.026vs1.241±0.102,P<0.05).裸鼠SMMC-7721细胞肺转移灶数目显著减少(62.5%vs100%,P<0.05).结论:ARG在体外可以抑制SMMC-7721细胞的黏附、侵袭和转移,在体内可以抑制肿瘤的转移.AIM：To evaluate the effects of treatment with arctigenin（ARG） on the adhesion,invasion,and metastasis of human hepatocellular carcinoma SMMC-7721 cells.METHODS：After SMMC-7721 cells were treated with ARG,cell adherence,migration,and invasion were assessed by MTT assay and Transwell migration assay.A model of pulmonary metastasis of SMMC-7721 cells was then generated in nude mice to evaluate the effect of arctigenin on tumor cell metastasis.RESULTS：The adhesion,migration,and invasion of SMMC-7721 cells were significantly inhibited after ARG treatment.Compared with control cells,the average reduced rates of adhe-sion,metastasis,and invasion in cells treated with ARG were 43.08%,55.19% and 58.21%,respectively.ARG inhibited the adhesion of SMMC-7221 cells in a concentration-and timedependent manner（72 h：0.260 ± 0.014 vs 0.999 ± 0.066,P 0.05;4 h：0.558 ± 0.026 vs 1.241 ± 0.102,P 0.05）.The number of metastatic pulmonary tumors in nude mice inoculated with arctigenin was lower than that in mice inoculated with control cells（62.5% vs 100%,P 0.05）.CONCLUSION：Treatment with arctigenin markedly inhibits the adherence,migration and invasion of SMMC-7721 cells.

关 键 词：牛蒡子苷元 肝癌 SMMC-7721细胞 黏附 侵袭 转移 

分 类 号：R735.7[医药卫生—肿瘤]