三种新型钙盐脱敏剂封闭牙本质小管的体外研究  

Occluding effects of three new calcium desensitizers on dentinal tubules in vitro

作　　者：熊正慧[1] 夏露[1] 梅蕾[2] 韩光政[1] 陈亚明[1] 

机构地区：[1]南京医科大学附属口腔医院修复科,南京医科大学口腔医学研究所210029 [2]香港大学菲腊牙科医院保存齿科

出　　处：《中华口腔医学杂志》2011年第4期214-217,共4页Chinese Journal of Stomatology

摘　　要：目的 体外比较3种新型钙盐脱敏剂封闭牙本质小管的效果,以期为临床应用提供参考.方法 新鲜离体第三磨牙25颗,制备成1.00 mm厚的牙本质薄片,6%柠檬酸处理后,按随机数字表法分为5组（n=5）,分别用蒸馏水（A组）、NovaMin（B组）、Pro-Argin（C组）、酪蛋白磷酸肽-无定形非晶体磷酸钙（D组）、75%NaF甘油（E组）处理样本冠面2 min,纵向劈开,扫描电镜观察牙本质表面和剖面后,测量牙本质小管封闭数目比及封闭深度,X射线能谱分析表面元素组成和剖面钙磷元素变化趋势.结果 A组牙本质小管完全开放,B、C、D、E组牙本质小管封闭数目比分别为（81.6±6.1）%、（71.5±6.3）%、（43.2±2.6）%、（39.9±4.1）%;剖面封闭深度分别为（30.33±2.26）、（24.55±2.58）、（16.10±4.65）、（8.90±1.51）μm;表面封闭数目比和剖面封闭深度4组间差异有统计学意义（P＜0.01）,但B、C组间差异无统计学意义（P＞0.05）;5组钙磷比差异无统计学意义（P=0.342）.结论 体外牙本质小管即刻封闭效果比较,NovaMin处理组在牙本质表面和剖面均显示出较好的封闭性能,可形成类牙本质样物质.Objective To evaluate the occluding effects of three new calcium desensitizers on dentinal tubules in vitro. Methods Twenty-five dentin specimens of 1.00 mm thick from freshly extracted third molars were divided into five groups randomly. After treatment with 6% citric acid, group A was treated with distilled water, and group B-E were treated with NovaMin, Pro-Argin, casein phosphopeptide-amorphous calcium phosphate（ CPP-ACP）, and 75% NaF glycerin for 2 min respectively. Then the teeth were vertically cleaved into two sections. The surfaces and cross sections were observed by the scanning electron microscope（SEM） and energy dispersive X-ray （EDX）, which were analyzed by the Image-Pro Plus software. Results The percentage of dentin tubules sealed were （ 81.6 ± 6. 1 ） %, （ 71.5 ± 6. 3 ） %, （43.2 ± 2. 6） %, （ 39. 9 ± 4. 1 ） %for groups B, C, D and E respectively, and the occluding depth were （ 30. 33 ± 2. 26 ）, （ 24. 55 ± 2. 58 ）,（16. 10 ±4. 65） and （8. 90 ± 1.51 ） μm respectively. The differences between these groups were statistically significant （P＜0. 01） except for group B and C （P ＞0.05）. However, there was no significant difference between all the groups for the Ca/P ratios （ P = 0. 342）. Conclusions NovaMin may have better immediate occluding effects than other desensitizers and could deposit a dentin-like mineral.

关 键 词：牙本质过敏 显微镜检查 电子 扫描 钙盐脱敏剂 

分 类 号：R781.2[医药卫生—口腔医学]