牛源金黄色葡萄球菌16S rRNA分子鉴定与随机多态性扩增分型  

Identification with 16S rRNA and RAPD typing of Staphylococcus aureus isolated from bovine mastitis

作　　者：邓海平[1] 蒲万霞[1] 梁剑平[1] 倪春霞[1] 孟晓琴[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所中国农业科学院新兽药工程重点开放实验室甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《中国兽医杂志》2011年第4期9-12,共4页Chinese Journal of Veterinary Medicine

基　　金：国家科技支持计划(2006BAD04A05);中央级公益性科研院所基本科研业务费专项资金项目(1610322011010)

摘　　要：利用16S rRNA保守序列对引起内蒙古地区奶牛乳房炎的金黄色葡萄球菌进行分离鉴定,并且建立引物随机多态性扩增(RAPD)体系对金黄色葡萄球菌分离株进行基因分型研究。结果表明,共分离出金黄色葡萄球菌35株,RAPD结果显示这35株菌均可得到清晰的RAPD指纹图谱,扩增产物在1～8条带之间,产物大小在350～4 500 bp之间。菌株分为6个基因型,Ⅳ型为该地区的流行优势菌群。不同牛场各基因型菌株分布有明显差异,这与牛场的环境和病原菌在牛场间流行传播情况有关。研究结果为地区性奶牛乳房炎的防治及新疫苗的研制提供了可靠的理论依据。The 16S rRNA conserved sequence was used for the identification of Staphylococcus aureus（S.aureus） strains causing bovine mastitis in Inner Mongolia area,and system of randomly amplified polymorphic DNA（RAPD） with a oligonucleotide primers OLP13 was established for research on genetic polymorphism of this S.aureus.The result show that 35 S.aureus strains were isolated and identified.All of the 35 S.aureus strains produced fingerprint distinctly and amplified out 1～8 electrophoresis strip with sizes ranging from 350～4 500 bp after RAPD.The S.aureus strains were separated to 6 genotype according to the fingerprint.The preponderant S.aureus strains in Inner Mongolia was TypeⅣ.Difference of environment and transmission of pathogenic bacteria was the main reason for the distinct discrepancy on proportion of different genotype strains in different cattle farms.These results provide a reliable theory evidence for preventive treatment and new bacteria research of regional bovine mastitis

关 键 词：奶牛乳房炎 金黄色葡萄球菌 16S RRNA RAPD 基因型 

分 类 号：S852.611[农业科学—基础兽医学]