椒苯酮胺对局灶性脑缺血再灌注损伤大鼠的神经保护作用  

Protective effects of piperphentonamine hydrochloride on cerebral ischemia reperfusion injury by middle cerebral ischemic injury(MCAO) in rats

作　　者：朱汉祎[1] 林焕冰[1,2] 陈玉嫔[1] 徐江平[1] 

机构地区：[1]南方医科大学药学院药理学系,广州510515 [2]广东省食品药品监督管理局审评认证中心,广州510080

出　　处：《军事医学》2011年第4期286-289,共4页Military Medical Sciences

基　　金：国家自然科学基金项目(30973518);广东省自然科学基金重点项目(7117782)

摘　　要：目的观察椒苯酮胺(piperphentonamine,PPTA)对大鼠脑缺血再灌注损伤后的保护作用,并探讨其作用机制。方法随机将SD大鼠分为假手术组、模型组、PPTA组(2.5,5,10 mg/kg)和阳性对照依达拉奉组(6 mg/kg)。采用大鼠大脑中动脉闭塞(MCAO)2 h再灌注模型,缺血1 h后静脉注射给药,复通灌流24 h后,采用Zea-Longa法进行神经功能缺陷评分,检测大鼠脑组织含水量,TTC染色法测定梗死体积及脑组织中SOD、MDA、GSH、NO、NOS生化指标。结果与模型组相比,PPTA组神经功能评分减低,梗死体积减少,脑组织中SOD、GSH活力增加,NOS活力降低,MDA和NO含量减少。其中以高剂量(10 mg/kg)组改变最为明显。结论 PPTA可能通过抑制脂质过氧反应和清除氧自由基等机制,发挥神经保护作用。Objective To test the neuroprotective effect of piperphentonamine hydrochloride（PPTA） and investigate the potential underlying mechanisms in focal cerebral ischemia-reperfusion in rats.Methods Sprague-Dawley rats were randomly divided into the sham group,the ischemia-reperfusion group,the PPTA treated group（2.5,5,and 10 mg/kg） and the edaravone treated group（6 mg/kg）.Rats were subjected to 24 h of reperfusion following ischemia for 2 h induced by middle cerebral artery occlusion（MCAO）.The different doses of PPTA were intravenously administered to rats 1 h after the onset of ischemia.The neurological behavioral test was performed 24 h after reperfusion by using the Zea-Longa scores.Infarction volumes were calculated by TTC-staining.The changes in water content,and the activity of SOD,GSH,NOS and the content of MDA and NO in brain tissue were measured.Results Compared with the ischemia-reperfusion group,the treatment with PPTA improved Zea-Longa scores,reduced infarction volume and brain water content.PPTA treatment also increased activities of SOD and GSH,decreased NOS activity,and reduced the levels of MDA and NO.The effect in high-dose group（10 mg/kg） was the most obvious in the present study.Conclusion This study suggests that the neuroprotective effects of PPTA are closely associated with inhibition of lipid peroxidation reaction and scavenging free radicals.

关 键 词：脑缺血/再灌注 椒苯酮胺 超氧化物歧化酶 丙二醛 谷胱甘肽 一氧化氮 一氧化氮合酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]