犬细小病毒北京分离株VP2基因克隆及变异分析  

Cloning and analysis of VP2 gene of canine parvovirus isolated from Beijing

作　　者：王净[1,2] 李英俊[3] 李刚[1] 史利军[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]河北北方学院动物科技学院,河北张家口075131 [3]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国兽医学报》2011年第5期649-653,共5页Chinese Journal of Veterinary Science

基　　金：中央级公益性科研院所基本科研业务费专项资金资助项目(2010JS-2;2009QN-3;0032007008);北京市科委基金资助项目(Z07010501780701)

摘　　要：对北京昌平地区分离的9株犬细小病毒(Canine parvovirus,CPV)进行VP2基因克隆测序,并利用生物信息学软件,将克隆的VP2基因与GenBank公布的国内外部分毒株序列相比较,绘制系统进化树,分析9株CPV VP2蛋白亲水性和抗原指数。结果表明,分离毒株与参考毒株核苷酸和氨基酸同源率在97%以上。通过序列比对与分析,确定其中有8株为New CPV-2a,1株为CPV-2,表明该地区目前主要以New CPV-2a流行为主。9株分离株在进化树上都能找到亲缘关系较近的毒株,没有形成新的分支。9株VP2蛋白亲水性高的区域抗原指数相应就高,不同株之间总体差别很小,主峰相同。The VP2 gene of canine parvovirus（CPV）,isolated from Changping district of Beijing,were amplified and cloned by PCR.Using biological information software,phylogenetic tree was constructed based on the VP2 genes of the cloned and others CPV strains from GenBank.Comparison results showed that the nucleotides and deduced amino acids sequence among CPV isolates to the references share beyond 97% homology.Eight of nine isolated strains belonged to New CPV-2a,and one of them belonged to CPV-2.The result showed that New CPV-2a was the main prevalent strain in Changping district of Beijing.The CPV isolated strains did not form obviously a typical cluster of China.The hydrophilicity and antigens index of the protein VP2 of the nine CPVs were the same of the main peak.

关 键 词：犬细小病毒 VP2基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]