山西猪源基因Ⅰ型乙型脑炎病毒的分离与鉴定  

Isolation of Japanese encephalitis virus(genotype Ⅰ) from swine in Shanxi province

作　　者：曹齐树[1] 钱平[1] 朱巧艳[1] 钱苏红 李静逸[1] 林飞宏[1] 李祥敏[1] 孙艳芳[1] 陈焕春[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,湖北武汉430070

出　　处：《中国兽医学报》2011年第5期692-696,702,共6页Chinese Journal of Veterinary Science

基　　金：国家重点基础研究发展计划("973"计划)资助项目(2010CB530103);农业部公益性行业研究专项资助项目(200803015);国家自然科学基金资助项目(30600447);华中农业大学自主科技创新基金资助项目(2009SC006)

摘　　要：利用RT-PCR技术从2009年山西运城某猪场疑似乙型脑炎仔猪脑组织扩增出乙型脑炎病毒pr M和E基因,采取病样通过BHK-21细胞传代,分离到1株病毒。间接免疫荧光和RT-PCR进一步证实该病毒为猪源乙型脑炎病毒,并命名为SX09S-01。根据pr M基因绘制的进化树表明SX09S-01毒株属于基因Ⅰ型,E基因与SA-14、SA-14-14-2、P3、HW和XJ69株的核苷酸同源性分别为88%,87%,88%,88%,98%,其蛋白具有强毒株典型特征。In this study,the prM and E fragments were amplified by RT-PCR from brains of piglets which were suspected to be infected with Japanese encephalitis virus（JEV） in Yunchen,Shanxi province 2009.A virus was isolated by passage in BHK-21 cells with brain tissues.The isolated virus was confirmed to belong to JEV by immunofluorescence and RT-PCR and named SX09S-01.The isolate belongs to genotype Ⅰ by the phylogenetic analysis based on prM gene.There were the homology of 88%,87%,88%,88% and 98% among the E genes of SA-14,SA-14-14-2,P3,HW,XJ69 and SX09S-01.E protein had some typical characteristics of strong virulent JEV.

关 键 词：猪 基因Ⅰ型乙型脑炎病毒 进化树 

分 类 号：S852.65[农业科学—基础兽医学] R535[农业科学—兽医学]