镰形棘豆对SMMC-7721肝癌细胞增殖及MMP-2表达的影响  

Influences of Oxytropis falcata on proliferation of SMMC-7721 Hepatocarcinoma cells and expression of MMP-2

作　　者：杨光明[1] 张芳芳[1] 王栋[1] 何媛媛[1] 童丽[2] 蔡宝昌[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室国家中医药管理局中药炮制标准重点实验室,江苏南京210029 [2]青海大学医学院,青海西宁810001

出　　处：《中国中药杂志》2011年第9期1227-1230,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30902012);江苏省中医药局项目(LB09026)

摘　　要：目的:观察镰形棘豆对人肝癌细胞SMMC-7721的增殖及其基质金属蛋白酶-2(MMP-2)分泌量的影响。方法:以不同质量浓度(25,50,100,250,500 mg.L-1)的镰形棘豆挥发油、总多糖、总黄酮、总生物碱和总皂苷部位处理SMMC-7721细胞24 h后,采用MTT法检测各部位对SMMC-7721细胞增殖的抑制作用;采用ELISA法测定挥发油部位和总黄酮部位处理SMMC-7721肝癌细胞24 h后细胞上清中MMP-2的分泌量;Real-time PCR法检测处理24 h后MMP-2 mRNA的转录情况。结果:MTT实验结果表明,挥发油和总黄酮部位抑制SMMC-7721细胞的增殖,并表现出一定的浓度依赖性。ELISA实验表明挥发油部位显著性地抑制MMP-2分泌量;Real-time PCR实验表明挥发油和总黄酮均显著抑制MMP-2 mRNA的表达。结论:镰形棘豆挥发油和总黄酮部位抑制人肝癌细胞SMMC-7721的增殖活性,可能与其下调MMP-2的分泌量和转录水平有关。Objective：To investigate the influences of extracts of Oxytropis falcata on proliferation of SMMC-7721 cells and expression of MMP-2（matrix metalloproteinase-2）.Method： SMMC-7721 cells were treated for 24 h with five fractions obtained from O.falcata in different concentrations.Inhibition on proliferation of the SMMC-7721 cells was assessed by MTT method.Secretion of MMP-2 was measured by ELISA in the supernatant of SMMC-7721 cells treated with fractions of essential oil and total flavonoids for 24 h.Transcription of mRNA of MMP-2 was detected by Real-time PCR.Result： In MTT assay,essential oil and total flavonoids showed potential antiproliferative activity on SMMC-7721 in a concentration dependent manner.Data of ELISA showed that fraction of essential oil suppressed secretion of MMP-2 significantly.Results of Real-time PCR indicated that both essential oil and total flavonoids restrained expression of mRNA of MMP-2.Conclusion： It suggested that essential oil and total flavonoids of O.falcata inhibit proliferation of SMMC-7721 cells through down-regulating secretion and expression of MMP-2 in cells.However,further experiments are necessary to carry out to investigate the potential mechanism.

关 键 词：镰形棘豆 SMMC-7721肝癌细胞 基质金属蛋白酶-2 

分 类 号：R735.7[医药卫生—肿瘤]