LC/MS法定性定量测定烟草中的1-氨基-1-脱氧-2-酮糖  

Qualitative and Quantitative Analysis of 1-Amino-1-deoxyketoses in Tobacco by High-Performance Liquid Chromatography Coupled with Mass Spectrometry

作　　者：翟玉俊[1] 黄伟[1] 陈华[1] 刘红海[1] 

机构地区：[1]甘肃烟草工业有限责任公司技术中心,兰州市南滨河中路1111号730050

出　　处：《烟草科技》2011年第4期58-61,共4页Tobacco Science & Technology

摘　　要：在合成1-脱氧-1-L-脯氨酸-D-果糖(Fru-Pro)和1-脱氧-1-L-丙氨酸-D-果糖(Fru-A la)的基础上,以Fru-Pro和Fru-A la作标样,建立了高效液相色谱-质谱联用技术(HPLC/MS)定性定量分析烟草中这2种物质的方法,并采用该方法测定了16个烟草样品。结果表明:①通过保留时间和SCAN模式下的质谱碎片能准确定性这2种物质,通过SIM模式选择正离子(m/z)278.0和252.0可分别定量Fru-Pro和Fru-A la;②Fru-Pro和Fru-A la的检出限分别为25.0和30.0μg/g,回收率93.1%和105.8%,RSD 4.23%和3.97%;③3个烤烟烟叶样品中的Fru-Pro和Fru-A la含量远远高于3个白肋烟叶样品,5个国产烤烟型卷烟样品中这2种物质的含量比1个进口烤烟型卷烟、2个国产和2个进口混合型卷烟样品都高。该方法适合烟草中这2种1-氨基-1-脱氧-2-酮糖的同时定性定量分析。Using the in-house synthesized N-（1-deoxy-D-fructos-1-yl）-L-proline（Fru-Pro） and N-（1-deoxy-D-fructos-1-yl）-L-alanine（Fru-Ala） as standards,a HPLC/MS method for qualitatively and quantitatively analyzing these two compounds in tobacco was developed.Sixteen tobacco samples were analyzed,the results showed that： 1） Fru-Pro and Fru-Ala were identifiable via retention time and main mass fragmentation under SCAN mode and quantifiable under selected positive ion mode at（m/z） 278.0 and（m/z） 252.0,respectively;2） The recoveries of Fru-Pro and Fru-Ala were 93.1% and 105.8% with the relative standard deviations of 4.23% and 3.97% and the limits of detection of 25.0 and 30.0 μg/g,respectively;3） The contents of Fru-Pro and Fru-Ala in 3 flue-cured tobacco leaf samples were much higher than that in 3 burley tobacco samples,in 5 domestic Virginian-type cigarette samples were higher than that in 1 imported Virginian-type cigarette sample,2 domestic and 2 imported blended-type cigarette samples.The method was suitable for the determination of these two compounds in tobacco samples.

关 键 词：1-氨基-1-脱氧-2-酮糖 MAILLARD反应 烟草 液相色谱/质谱法 

分 类 号：TS411.2[农业科学—烟草工业]