脑出血后多聚二磷酸腺苷核糖聚合酶过度表达与脑水肿的相关性  

Poly（ADP-ribose） polymerase overexpression and brain edema formation after intracerebral hemorrhage in rats

作　　者：吴刚[1] 鲍旭辉[1] 胡枢坤[1] 黄峰平[1] 

机构地区：[1]复旦大学附属华山医院神经外科上海市神经外科临床医学中心,上海200040

出　　处：《中华实验外科杂志》2011年第5期740-742,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30571905）

摘　　要：目的探讨血红蛋白导致脑出血（ICH）后脑水肿的机制。方法将血红蛋白注入sD大鼠的右侧基底节区，24h后将其处死取脑组织检测脑水含量及钠离子含量。同时，采用Westernblot分析及免疫组织化学方法来检测多聚二磷酸腺苷核糖聚合酶（PARP）的表达，并观察铁螯合剂——去铁敏（DFX）对PARP表达的影响。结果脑内注射血红蛋白后24h发现注射侧脑水含量明显升高[基底节含水量由（78．7±0．3）％上升至（82．1±1．2）％]，同时PARP表达上调；而在脑内注射血红蛋白同时腹腔注射500mg／kg的去铁敏则能使PARP表达下调并且使脑水肿减轻[基底节含水量由（82．1±1．2）％降至（80．4±1．0）％]。结论在脑出血后血红蛋白诱导的迟发性脑水肿的形成过程中，PARP起到了潜在的重要作用；而螯合血红蛋白的降解产物铁并抑制PARP过度表达，有可能成为减轻脑出血后脑水肿的新的有效治疗方法。Objective To explore the mechanism of brain edema formation caused by hemoglobin after intracerebral hemorrhage （ICH）. Methods Hemoglobin was infused into the right basal ganglia of SD rats with stereotaetic guidance. The animals were killed 24 h later for detection of brain water and ion contents. Western blotting and immunohistochemistry were applied to detect the expression of poly（ ADP-ribose） polymerase （PARP）. The effect of the iron chelator deferoxamine （DFX） on PARP expression was also examined. Results Intracerebral infusion of hemoglobin caused an increase in brain water content [from （78.7 ±0.3）% to （82.1 ± 1.2）% in ipsilateral basal ganglia] at 24th h. At the same time, PARP expression was up-regulated after hemoglobin infusion. By the intraperitoneal injection of 500 mg/kg DFX, brain edema induced by hemoglobin was attenuated significantly [ from （ 82. 1 ± 1.2） % to （ 80.4 ±1. 0）% in ipsilateral basal ganglia~, and PARP expression was also down-regulated. Conclusion PARP might play a potential role, at least in part, in delayed edema development after ICH, and reducding iron production by iron chelator and down-regulating PARP overexpression might be a useful target for therapeu- tic intervention to limit brain edema after ICH.

关 键 词：脑出血 脑水肿 血红蛋白 铁 

分 类 号：R743.34[医药卫生—神经病学与精神病学]