HPLC法同时测定杠板归中阿魏酸与香草酸的含量  

Content Determination of Ferulic Acid and Vanilla Acid in Polygonum perfoliatum by HPLC

作　　者：黄家宇[1] 李莉[1] 刘青[1] 盛钰[1] 罗天军 刘豫[1] 

机构地区：[1]贵阳医学院药学院,贵阳市550004 [2]贵州远程制药有限责任公司,贵阳市550018

出　　处：《中国药房》2011年第19期1783-1784,共2页China Pharmacy

基　　金：国家科技支撑计划子课题(2009BAI74B02-4)

摘　　要：目的:建立同时测定杠板归中阿魏酸与香草酸含量的方法。方法:采用高效液相色谱法。色谱柱为Diamonsil C18(250mm×4.6mm,5μm),流动相为1.0%甲酸-甲醇(40∶60),流速为1.0mL·min-1,检测波长为290nm。结果:阿魏酸进样量的线性范围为0.6～3.0μg(r=0.9995),平均回收率为99.32%,RSD=1.06%(n=9);香草酸进样量的线性范围为0.454～2.270μg(r=0.9999),平均回收率为99.92%,RSD=2.17%(n=9)。结论:本方法灵敏、准确、重复性好,可用于杠板归药材的质量控制。OBJECTIVE：To develop the method for the simultaneous determination of ferulic acid and vanillic acid in Polygonum perfoliatum. METHODS：HPLC method was adopted. The determination was performed on Diamonsil C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of 1.0% formic acid-methanol（40 ∶ 60） at flow rate of 1.0 mL·min-1. The detection wavelength was set at 290 nm. RESULTS：The linear range of ferulic acid was 0.6～3.0 μg（r=0.999 5） with an average recovery of 99.32%（RSD=1.06%,n=9） . The linear range of vanilla acid was 0.454～2.270 μg（r=0.999 9） with an average recovery of 99.92%（RSD=2.17%,n=9） . CONCLUSION：The method is sensitive,accurate and reproducible for the quality control of P. perfoliatum.

关 键 词：杠板归 阿魏酸 香草酸 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]