NaCl胁迫对藜幼苗叶片渗透调节物质和保护酶的影响  

Effects of NaCl Stress on Osmotic Adjustment Solute and Protective Enzyme in Seedling Leaves of Chenopodium album L.

作　　者：施剑杰[1] 金慧丽[1] 孙存华[1] 孙东[1] 李百伟[1] 王丹[1] 

机构地区：[1]徐州师范大学生命科学学院,江苏徐州221116

出　　处：《安徽农业科学》2011年第12期6943-6946,共4页Journal of Anhui Agricultural Sciences

基　　金：徐州师范大学自然科学基金重点项目(06XLA11);徐州师范大学自然科学基金预研项目(08XLY06)

摘　　要：[目的]研究不同浓度NaCl胁迫对藜叶片中渗透调节物质和保护酶活性的影响,以探讨藜的耐盐机理,为藜的人工栽培及推广提供理论依据。[方法]待藜幼苗长到4叶期时,用0%、0.6%、1.2%、1.8%NaCl溶液进行根部胁迫处理,分别于处理0、1、3、5、7 d后从藜幼苗上部取第3～5片功能叶片进行各项生理指标的测定。[结果]在0.6%NaCl胁迫下,渗透调节物质(可溶糖、脯氨酸和可溶性蛋白质)含量和保护酶(SOD、POD和CAT)活性都是缓慢增加的,丙二醛含量增加不大,膜脂过氧化水平低。在1.2%、1.8%NaCl胁迫下,渗透调节物质(可溶性糖、脯氨酸和可溶性蛋白质)含量随胁迫时间的延长而增加,SOD、POD、CAT活性随胁迫时间的延长先升高后降低,丙二醛含量显著提高,膜脂过氧化水平增大。[结论]藜可以通过渗透调节物质增加和保护酶活性增大来对适度的盐胁迫有一定的适应性,但高浓度、长时间的盐胁迫会对藜造成严重损伤。[Objective] Chenopodium album L.were stressed by different concentration NaCl and identified of osmotic adjustment solute and protective enzyme activity in order to lay a foundation for studying clarifying the salt-tolerant physiological mechanism of Chenopodium album L.and supplied theoretical basis and practical guidance for artificial cultivation and popularization of C.album.[Method] C.album seedlings grown to 4th leaf stage were treated by NaCl stress with concentrations of 0%,0.6%,1.2% and 1.8% and the various physiological indexes of the 3th-5th function leaves in upper plant were determined after treating for 0,1,3,5 band 7 days.[Result] Under stress with 0.6% NaCl,the osmotic adjustment solute content（the soluble sugar,proline,the soluble protein） and protective enzyme activities（SOD,POD,CAT） in seedling leaves of C.album increased slowly and the change of MDA content was not apparent.Under 1.2%,1.8% NaCl stress treatment,the osmotic adjustment solute content increased by the extension of NaCl stress time（the soluble protein increased unremarkably）,and the activities of SOD,POD,CAT were increased at first and then decreased by the extension of NaCl stress time,however the MDA increased remarkably.[Conclusion] These indicated that C.album had some adapt ability to moderate NaCl osmotic stress by regulating the internal osmotic adjustment solute and activities of defense enzymes,but the NaCl osmotic stress with high concentration and long time might lead to severe damage on C.album.

关 键 词：藜 NACL胁迫 渗透调节物质 保护酶 

分 类 号：S188[农业科学—农业基础科学]