藏绵羊脂蛋白脂酶基因克隆及序列分析  

Tibetan Sheep LPL Gene Clone and Bioinformatic Analysis

作　　者：高思[1] 徐亚欧[1] 毛亮[2] 邵欢欢[3] 杨虎林[4] 舒浩国 

机构地区：[1]西南民族大学,四川成都610041 [2]泸州医学院,四川沪州646000 [3]四川大学,四川成都610041 [4]四川省阿坝州阿坝县畜牧局,四川阿坝624600

出　　处：《安徽农业科学》2011年第12期7291-7294,共4页Journal of Anhui Agricultural Sciences

基　　金：四川省应用基础研究重点项目(07JY029-045)

摘　　要：[目的]为深入研究藏绵羊肉用性能的遗传调控与营养代谢关系。[方法]利用RT-PCR和T-A克隆技术获得了藏绵羊LPL基因,并对其进行生物信息学分析。[结果]藏绵羊LPL编码基因全长1437 bp,编码478个氨基酸。将藏绵羊LPL基因及氨基酸序列分别与GenBank中公布的11种动物进行序列一致率比对,发现藏绵羊与所选动物的LPL基因序列一致率在84.6%～99.6%,LPL氨基酸序列一致率在88.8%～99.0%。藏绵羊与普通绵羊LPL基因存在6个位点核苷酸差异,其中有一个核苷酸位点的差异没有引起相应氨基酸的改变,其余5个位点核苷酸的不同都引起了氨基酸的差异。[结论]该研究可为了解LPL基因的演化关系及作用机理提供资料。[Objective]The aim was to deeply study the relationship between the genetic regulation of meat performance of Tibetan sheep and nutrition and metabolism.[Method] The LPL coding gene of Tibetan sheep was cloned by reverse-translation PCR and T-A clone technology,then it was analyzed by Bioinformatics software.[Result] The results showed that LPL gene of Tibetan sheep contained 1437 bp nucleotides and encoded 478 amino acids.The multiple sequence alignment such as Tibetan sheep,sheep,goat,cattle,yak,pig,dog,cat,baboon,orangutan,human,Norway rat and rattus showed that the total homologous rate of LPL gene was 84.6%～99.6%,and the homologous rate of amino acids was 88.8%～99.0%.Moreover,6 different nucleotides were found between Tibetan sheep and common sheep.One of these nucleotide was synonymous codon so that the amino acid which the synonymous codon encoded was identical between Tibetan sheep and common sheep,and the other five nucleotides which encoded different amino acids between Tibetan sheep and common sheep.[Conclusion] The study can provide reference for knowing the evolution relation of LPL gene and its mechanism of action.

关 键 词：藏绵羊 脂蛋白脂酶 克隆 序列分析 系统进化 

分 类 号：S188.1[农业科学—农业基础科学]