7种常用渔药及组合对致病性鮰爱德华氏菌的体外抗菌试验  

Antibacterial Trial of Seven Fishery Drugs and Their Combinations against Pathogenic Edwardsiella ictaluri from Channel Catfish In Vitro

作　　者：张彬[1] 黄婷[1] 陈福艳[1] 陈明[1] 余晓丽[1] 梁万文[1] 

机构地区：[1]广西水产研究所鱼病研究室,广西南宁530021

出　　处：《西南农业学报》2011年第2期761-765,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2008BADB9B04);广西科学研究与技术开发计划项目(桂科合0718007B-34)

摘　　要：为探讨7种常用抗菌渔药对致病性鮰爱德华氏菌的体外抗菌作用,采用2倍稀释法测定了7种渔药对致病菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),在初筛MIC的基础上,以MIC、5倍MIC、10倍MIC和20倍MIC下平板抑菌圈直径为参数,分别比较5种药物抗菌效果并优化最佳参数;利用优化的最佳参数比较5种药物单独用药与联合用药对鮰爱德华氏菌的抑菌效果。结果表明,抗菌先锋对鮰爱德华氏菌的MIC和MBC最小,分别为3.2和6.4μg/mL,其次是伯乐立康,分别为12.8和25.6μg/mL,而鱼康和肠炎烂鳃灵的浓度为1638.4μg/mL时仍无抗菌效果;5倍MIC下抑菌圈直径为筛选抗菌药物的最佳参数,5倍MIC下,鮰爱德华氏菌对抗菌先锋高度敏感,对伯乐立康、菌必清、菌毒康和海鱼安中度敏感;菌必清+抗菌先锋的联合抗菌效果最佳。The present study was conducted to research the antibacterial effects of 7 common fishery drugs and their combinations against pathogenic Edwardsiella ictaluri from channel catfish in vitro.The minimum inhibitory concentrations and the minimal bactericidal concentrations of 7 fish drugs were tested by the method of doubling dilution in test tube.On the basis of preliminary screening,antibacterial effecst of 5 fish drugs against Edwardsiella ictaluri were compared and the optimal parameters were optimized,respectively,by using the circle of inhibiting bacterium of 1,5,10 and 20 times MICs as parameters,MIC regarded as a unit.Then,the best parameter was applied to compare the inhibiting effects of 5 fishery drugs in single and their combinations.The results showed that the MIC and MBC of ETSP was the minimum,which were 3.2 and 6.4 μg/mL,respectively,and that of GCHBH were 12.8 and 25.6 μg/mL,respectively,but CSP and NSP had no inhibitory effects on it,even at the concentration of 1638.4 μg/mL.The bacteriostasis circle under 5 times concentration of MIC was the best parameter.Under the parameter,Edwardsiella ictaluri was the most sensitive to ETSP,and followed by GCHBH,EP,FP and DHP.The combination of EP and ETSP had the best antibacterial effect.

关 键 词：鮰爱德华氏菌 斑点叉尾鮰 渔用药物 体外抗菌作用 

分 类 号：S965[农业科学—水产养殖]