果叶兼用多倍体新桑品种嘉陵30号多酚氧化酶(PPO)酶学特性研究  

Study on the Enzymological Characteristics of PPO in Jialing 30-a New Polyploid Mulberry(Morus L.) Variety for Leaf-and Fruit-Production

作　　者：王茜龄[1] 刘树擎[1] 李镇刚[1] 金筱耘[1] 余茂德[1] 

机构地区：[1]西南大学生物技术学院,重庆400716

出　　处：《蚕学通讯》2011年第1期15-19,共5页Newsletter of Sericultural Science

基　　金：国家蚕桑产业技术体系建设项目

摘　　要：本文以人工四倍体新桑品种嘉陵30号为供试材料,以邻苯二酚为作用底物,用紫外分光光度吸收法,测定不同pH、反应温度、底物浓度等对多酚氧化酶活性的影响。实验结果表明,嘉陵30号的多酚氧化酶对光的最大吸收波长为410nm,PPO酶活力为0.032nm/min,最适底物浓度为均0.4mol/L,最适pH为4.0,最适温度为25℃。多酚氧化酶浓度在0.2-0.4体积范围内,催化反应的速度逐渐加快,抑制剂对多酚氧化酶活性的总体影响为:抗坏血酸>Na2SO3>EDTA>柠檬酸。The effects of pH,reaction temperature and substrate（catechol） concentration on the activity of polyphenol oxidase（PPO） in Jialing 30,an artificial tetraploid of mulberry,were determined spectrophotometrically.The results showed that the maximum absorption wavelength of PPO of Jialing 30 was 410 nm,the PPO activity was 0.032 nm/min,and the optimum substrate concentration,pH and temperature for it were 0.4 mol/L,4.0 and 25℃,respectively.With a PPO concentration of 0.2-0.4,the reaction rate became accelerated.The effects of inhibitors on PPO activity appeared in the order of vitamin CNa2SO3EDTAcitric acid.

关 键 词：桑树 嘉陵30号 多酚氧化酶(PPO) 酶学特性 

分 类 号：S888[农业科学—特种经济动物饲养] S663.9[农业科学—畜牧兽医]