食管鳞状细胞癌组织中FAK、Paxillin和MMP-9蛋白的表达及临床意义  

Expression and clinical significance of FAK,Paxillin and MMP-9 protein in esophageal squamous cell carcinoma tissue

作　　者：庞霞[1] 李晟磊[1] 赵志华[1] 赵阿红[1] 张红新[1] 高冬玲[1] 

机构地区：[1]郑州大学附属第一医院病理科,450052

出　　处：《重庆医学》2011年第14期1367-1369,I0001,共4页Chongqing medicine

基　　金：河南省基础与前沿技术研究项目(082300453202)

摘　　要：目的探讨FAK、Paxillin和MMP-9蛋白在食管鳞状细胞癌(ESCC)组织中的表达及其意义。方法应用免疫组化SP法检测59例ESCC组织、27例癌旁不典型增生组织及36例健康食管黏膜组织中FAK、Paxillin及MMP-9蛋白的表达,并分析3种蛋白与临床病理学参数之间的关系。结果 FAK、Paxillin及MMP-9蛋白在ESCC组织中的表达均明显高于癌旁不典型增生组织和正常食管黏膜组织,差异均有统计学意义(P<0.05)。FAK和Paxillin蛋白表达与ESCC的组织学分级、浸润深度及淋巴结转移密切相关(P<0.05),MMP-9蛋白表达与癌的组织学分级及淋巴结转移密切相关(P<0.05);进一步相关性分析表明,MMP-9与FAK、Paxillin蛋白表达呈正相关关系(r值分别为0.299和0.464,P值分别为0.022和0.000)。结论 FAK、Pax-illin及MMP-9的高表达可能在食管鳞癌的浸润转移中发挥重要作用。Objective To explore the expressions of FAK,Paxillin and MMP-9 proteins in esophageal squamous cell carcinoma（ESCC）tissues and their significances.Methods Immunohistochemical SP method was used to detect the expressions of FAk,Paxillin and MMP-9 proteins in 59 cases of ESCC tissues,27 cases of adjacent atypical hyperplasia epithelium and 36 cases of normal esophageal epithelium,and the relationships between three proteins above and clinicopathological features were analyzed.Results The protein expression levels of FAK,Paxillin and MMP-9 in ESCC tissues were significantly higher than those in the adjacent atypical hyperplasia and normal mucous tissues,and there was significant difference（P0.05）.The expressions of FAK and Paxillin proteins were both closely correlated with the tumor grade,infiltrative depth and lymph node metastasis in ESCC tiissues（P0.05）,whereas the expression of MMP-9 protein was tightly correlated with the tumor grade and lymphatic metastasis in esophageal squamous cell carcinoma（P0.05）.Further,correlation analysis revealed that expressions of FAK and Paxillin proteins were both positively related to the expression of MMP-9 protein（P0.05）（r=0.299 and 0.464,P=0.022 and 0.000,respectively）.Conclusion Overexpressions of FAk,Paxillin and MMP-9 proteins may play pivotal roles in the invasiveness and metastasis of ESCC,which can be achieved via the regulation of MMP-9 expression.

关 键 词：食管 肿瘤 鳞状细胞 黏着斑激酶 桩蛋白 基质金属蛋白酶9 肿瘤转移 浸润 

分 类 号：R735.1[医药卫生—肿瘤]