牛心内膜腺苷三磷酸双磷酸酶对血小板聚集的抑制作用(英文)  

Inhibition of platelet aggregation by bovine endocardial apyrase

作　　者：易富贤[1] 黄韶玲[1] 郭迅[1] 孙平[1] 郭兆贵[1] 

机构地区：[1]湖南医科大学分子药理研究室,长沙中国410078

出　　处：《中国药理学报》1999年第9期851-854,共4页Acta Pharmacologica Sinica

摘　　要：目的:研究牛心内膜内皮细胞(EEC)腺苷三磷酸双磷酸酶的抗血小板聚集效应。方法:培养牛EEC。以高效液相色谱法测定ADP,用比浊法测血小板聚集。结果:ADP 500μmol·L^(-1)与EEC温孵后,引起ADP浓度进行性地降低。与此相适应的是,未代谢的ADP诱导血小板聚集的能力也降低。在阿司匹林处理过的EEC 1×10~9cells·L^(-1)存在下,凝血酶500U·L^(-1)及PAF 1nmol·L^(-1)诱导的经阿司匹林1mmol·L^(-1)及亚甲基蓝10μmol·L^(-1)处理过的血小板聚集明显受到抑制,且聚集是可逆的;EEC的此种作用类似于腺苷三磷酸双磷酸酶的作用。EEC明显抑制ADP 5μmol·L^(-1)诱导的血小板聚集,但不能抑制ADP-β-S 15μmol·L^(-1)诱导的血小板聚集。结论:腺苷三磷酸双磷酸酶水解ADP是牛EEC重要的抗血栓机制。AIM: To study the anti-aggregatory effect of bovine endocardial endothelial cell (EEC)-associated apyrase. METHODS: Cultured bovine EEC was used. Adenosine diphosphate ( ADP) was analyzed by reversed phase HPLC, and rabbit platelet aggregation was measured turbimetrically. RESULTS: Incubation of EEC with ADP 500 μmol·L-1 resulted in a progressive decrease in ADP concentration, which was paralleled by the decrease in platelet aggregating potential of the unmetabolized ADP. In the presence of aspirin (Asp 1 mmol·L-1)-treated EEC 1×109 cells ·L-1, the aggregation of Asp (l mmol·L-1) and methylene blue (10 μmol·L-1) -treated platelets in response to thrombin 500 U·L-1 and platelet activating factor (PAF 1 nmol·L-1) was markedly inhibited and was reversible, which was very similar to that in apyrase-treated platelets. The supernatants of EEC had no effect on platelet aggregation. EEC inhibited ADP (5 μmol·L-1)-induced platelet aggregation, but failed to inhibit adenosine 5'-O-(2-thiodiphosphate) (ADP-β-S, an unmetabolizable structural analog of ADP, 15 μmol·L-1)-induced platelet aggregation. CONCLUSION: ADP hydrolysis by EEC-associated apyrase is a major anti-thrombotic mechanism of bovine EEC.

关 键 词：腺苷三磷酸 双磷酸酶 心内膜 血小板聚集 

分 类 号：R973[医药卫生—药品]