大豆异黄酮微波干法辅助提取及其机理研究  

Extraction of soybean isoflavone by microwave radiation without solvent and its mechanism

作　　者：彭游[1,2,3] 邓泽元[2] 叶志刚[1] 喻国贞[1] 

机构地区：[1]九江学院化工学院,江西九江332005 [2]南昌大学食品科学与技术国家重点实验室,南昌330047 [3]江西省鄱阳湖生态经济研究中心,江西九江332005

出　　处：《广西植物》2011年第2期266-269,238,共5页Guihaia

基　　金：江西省自然科学基金(2010GZN0106);江西省教育厅科技项目(GJJ08440;GJJ11626)~~

摘　　要：考察大豆异黄酮的微波光波组合无溶剂提取方法,发现微波光波提取干法仅用9.4%的N,N-二甲基甲酰胺DMF作为能量传递介质,功率800 W(微波55%与光波45%)加热6 min后,乙醇萃取得总黄酮。大豆苷元的提取率为0.14%,与相应的常规提取法接近,该法有操作简单快速,成本低,环境污染小等优点。利用高倍荧光显微镜FM,IR对微波提取机理进行初步研究表明,微波光波可能是从对植物组织结构的影响上来改善次生代谢产物的提取效率。Extraction of the soybean isoflavone was studied by combination method of microwave and light wave radiation without solvent.We discovered that only using 9.4% N,N-dimethyl formamide DMF as the energy transfer medium,heating the sample for 6min with power 800W（microwave 55% and light wave 45%）,the total flavanone was extracted with ethyl alcohol.The extraction rate of daidzein that was 0.14%,closed with the corresponding conventional extraction process.This method had many merits,such as simplicity of operator,rapid,low cost,small environmental pollution and so on.Extraction mechanism was investigated preliminarily with high magnification fluorescence microscope FM,IR.It indicated that the improvement of extraction efficiency of secondary metabolite was possibly because the plant tissue structure was changed by microwave.

关 键 词：大豆异黄酮 微波光波 提取机理 干法 

分 类 号：Q946[生物学—植物学]