X线照射对人结直肠癌细胞株SW480中Kiss-1基因表达的影响  

The Influence of X-Ray Radiation on Expressions of Kiss-1 Gene in Human Colorectal Cancer Cell Line SW480

作　　者：涂明美[1] 陈绍勤[1] 戴起宝[1] 柯春林[2] 林素勇[1] 

机构地区：[1]福建医科大学附属第一医院胃肠外科,福建福州350005 [2]福建医科大学附属第一医院放疗科,福建福州350005

出　　处：《肿瘤基础与临床》2011年第2期95-97,共3页journal of basic and clinical oncology

基　　金：福建省自然科学基金项目(编号:2008J0274);福建省卫生厅青年科研课题项目(编号:2009-1-12)

摘　　要：目的探讨X线照射对体外培养人结直肠癌细胞株SW480中肿瘤转移抑制基因Kiss-1表达的影响。方法应用SYBRGreen实时定量PCR检测、相对定量法分析不同剂量X线照射后24、48 h SW480中Kiss-1 mRNA的表达水平。结果与对照组相比,照射后24 h,各照射组Kiss-1 mRNA表达的差异均无统计学意义(P>0.05);照射后48 h,2、4、6 Gy组Kiss-1 mRNA表达上调(P<0.05),8 Gy组Kiss-1 mRNA的表达差异无统计学意义(P>0.05)。结论 X线照射不会导致结直肠癌细胞Kiss-1基因表达下调,放疗可能不会增加结直肠癌转移的风险。Objective To investigate the influence of X-ray radiation on Kiss-1 gene expressions of colorectal cancer cell line SW480 in vitro.Methods SW480 was irradiated at different doses.The expression differences of Kiss-1 mRNA were detected using SYBR Green quantitative real-time PCR at 24,48 h.Then the relative quantification method was used to analyze the differences.Results For the Kiss-1 gene expressions of SW480,there was no significant difference 24 h after radiation（P0.05）.However,after radiation with 2,4,6 Gy for 48 h,the Kiss-1 mRNA expressions were increased（P0.05）,but there was no significant difference for 8 Gy group（P0.05）.Conclusion Radiation can′t decrease Kiss-1 gene expressions of colorectal cancer cells,and the radiotherapy can′t increase the risk of colorectal cancer metastases.

关 键 词：结直肠肿瘤 电离辐射 肿瘤转移 KISS-1 聚合酶链反应 

分 类 号：R735.3[医药卫生—肿瘤] R730.55[医药卫生—临床医学]