磁性附着体模拟静磁场对成骨细胞分化功能的影响  

The effects of simulating static magnetic fields of magnetic attachments on the differentiation of osteoblasts

作　　者：赵煜[1] 李冰雁[2] 杜莉[3] 巢永烈[3] 

机构地区：[1]中山大学光华口腔医学院附属口腔医院口腔医学研究所,广州510055 [2]南方医科大学珠江医院口腔科 [3]四川大学华西口腔医院修复科

出　　处：《中华口腔医学研究杂志（电子版）》2011年第2期24-27,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省自然科学基金博士启动项目(9451008901002644)

摘　　要：目的研究不同强度的磁性附着体模拟静磁场对成骨细胞分化功能的影响。方法利用细胞静磁场加载装置,对体外培养的SD大鼠成骨细胞分别进行12.5、125和250mT的静磁场加载,连续加载1、3、5和7d,同时设不加载静磁场的对照组,采用放射免疫测定技术,检测成骨细胞培养上清液骨钙素(OCN)含量。结果静磁场加载1和3d后,各磁场处理组OCN活性和对照组相比差异无统计学意义(P>0.05)。静磁场加载5和7d后,12.5、125和250mT磁场处理组成骨细胞分泌OCN的活性明显升高,与对照组相比差异有统计学意义(P<0.05),且保持较高水平。但各磁场处理组之间差异无统计学意义(P>0.05)。结论在本试验条件下,一定强度静磁场可以促进成骨细胞合成骨钙素的功能,促进成骨细胞分化。Objective To explore the biological effects of static magnetic fields （SMF） of dental magnetic attachment system with different intensities on osteocalcin （OCN） activities of osteoblasts. Methods In this study, the cultured rat osteoblasts were exposed continuously to 12.5 mT, 125 mT, and 250 mT SMF for one, three, five, and seven days, respectively. While, controls were also set up free of SMF. The activities of cellular OCN were examined by radioimmunoassay and statistical analysis was performed. Results Osteoblasts exposed to 12.5 mT, 125 mT, and 250 mT SMF for one day and three days did not exhibit significant differences on OCN activities, as compared to those of the control group （P 0.05）. However, when the SMF exposure continued to five days and seven days, the cellular OCN activities of the test groups were significantly higher （P 0.05）. And no difference was found between the three SMF exposure groups （P 0.05）. Conclusions Under this experimental condition, the static magnetic fields of magnetic attachments can stimulate OCN activities of osteoblasts, as to enhance the differentiation of osteoblasts.

关 键 词：磁性附着体 静磁场 成骨细胞 骨钙素 

分 类 号：R783.6[医药卫生—口腔医学]