Identification of four SNPs and association analysis with meat quality traits in the porcine Pitx2c gene  

作　　者：WU WangJun ZUO Bo REN ZhuQing HAPSARI A.A.R LEI MingGang XU DeQuan LI FengE XIONG YuanZhu 

机构地区：[1]Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture and Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China [2]Faculty of Animal Agriculture, Diponegoro University, Tembalang Campus-Semarang 50275, Indonesia

出　　处：《Science China(Life Sciences)》2011年第5期426-433,共8页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China (Grant No.2006CB102102);National High Technology Research and Development Program (Grant No.2006AA10Z1D6);National Natural Science Foundation of China (Grant No.30800782);New Teacher Research Fund for the Doctoral Program of Higher Education (Grant No.20070504064)

摘　　要：The association of the porcine Pitx2c gene with meat quality traits was investigated in the present study. A total of eight single nucleotide polymorphisms （SNPs） were found. Allele frequencies of four SNPs were further detected in four commercial breeds and eight Chinese indigenous breeds. Single SNP and meat quality associations were analyzed in a YorkshirexMeishan F2 population. The SNPs c.474C〉T （P〈0.01） and c.636C〉T （P〈0.05） showed a significant association with meat color （MCV1）. The SNPs c,＊37G〉A and c.＊47G〉A were significantly associated with drip loss rate （DLR）, water holding capacity （WHC） and meat color value （MCV1） consistently （P〈0.05）. Linkage disequilibrium （LD） analysis revealed that the adjacent SNPs were in LD. Two major haplotypes were identified, and association analysis between haplotype combinations and meat quality indicated that the presence of two copies of haplotype 1 -CCGG- may improve meat quality.The association of the porcine Pitx2c gene with meat quality traits was investigated in the present study.A total of eight single nucleotide polymorphisms (SNPs) were found.Allele frequencies of four SNPs were further detected in four commercial breeds and eight Chinese indigenous breeds.Single SNP and meat quality associations were analyzed in a Yorkshire×Meishan F 2 population.The SNPs c.474C>T (P<0.01) and c.636C>T (P<0.05) showed a significant association with meat color (MCV1).The SNPs c.37G>A and c.47G>A were significantly associated with drip loss rate (DLR),water holding capacity (WHC) and meat color value (MCV1) consistently (P<0.05).Linkage disequilibrium (LD) analysis revealed that the adjacent SNPs were in LD.Two major haplotypes were identified,and association analysis between haplotype combinations and meat quality indicated that the presence of two copies of haplotype 1-CCGG-may improve meat quality.

关 键 词：association HAPLOTYPE Pitx2c meat quafity PIG 

分 类 号：S828.2[农业科学—畜牧学] Q987[农业科学—畜牧兽医]