马钱子碱对多发性骨髓瘤U266细胞JAK-STAT信号通路的影响  

Effects of Brucine on Multiple Myeloma U266 Cell JAK-STAT Signaling Pathway

作　　者：冯静[1] 马艳萍[1] 

机构地区：[1]山西医科大学第二医院血液内科,山西太原030001

出　　处：《中国现代医生》2011年第13期1-2,9,共3页China Modern Doctor

基　　金：山西省留学人员择优资助项目(晋留管办发[2008]10号99合作项目)

摘　　要：目的观察马钱子碱对多发性骨髓瘤U266细胞JAK-STAT通路的影响。方法马钱子碱和/或JAK-STAT途径特异性抑制剂AG490培养多发性骨髓瘤U266细胞,MTT法检测马钱子碱对多发性骨髓瘤U266细胞株增殖的影响,计算IC50值,用于实验。RT-PCR法检测马钱子碱对多发性骨髓瘤U266细胞stat3、stat5 mRNA表达的影响。结果 (1)马钱子碱对多发性骨髓瘤细胞增殖的抑制率呈剂量和时间依赖性。(2)马钱子碱AG490均可下调stat3、stat5 mRNA表达水平(P<0.05),而马钱子碱作用强于AG490组,并呈时间依赖性。马钱子碱+AG490组作用强于马钱子碱、AG490单独作用组,呈时间依赖性。结论马钱子碱能下调stat3、stat5 mRNA表达水平,抑制细胞JAK-STAT信号转导通路,从而抑制多发性骨髓瘤U266细胞增殖。Objective To observe the brucine on multiple myeloma U266 cell JAK-STAT pathway. Methods Strychnine and/or JAK-STAT pathway inhibitor AG490 multiple myeloma U266 cell culture,MTT assay of brucine on the multiple myeloma cell line U266 impact of value-added,calculate IC50 values,for experiment. RT-PCR detection of strychnine on the onset of myeloma U266 cells stat3,stat5 mRNA expression. Statistical methods of two groups of comparisons using t-test,multiple compares the single factor analysis of variance. Results （1）Brucine on the multiple myeloma cell proliferation rate in a dose and time dependent.（2）Brucine and AG490 can be reduced stat3 ,stats mRNA expression level （P〈0.05）and brucine stronger than that of AR490 group,and time-depen- dent manner. Brucine AG490 group was stronger than strychnine,AG490 alone group,time-dependent manner. Conclusion Brucine＂ could down stat3,stat5 mRNA expression,inhibition of JAK-STAT signal transduction pathway,thereby inhibiting the proliferation of multiple myeloma U266 cells.

关 键 词：马钱子碱 JAK-STAT通路 增殖 AG490 

分 类 号：R733.3[医药卫生—肿瘤]