弥漫大B细胞淋巴瘤CARMA1基因与多发性骨髓瘤癌基因蛋白表达的关系及其意义  

Correlation between the expressions of CARMA1 gene and MUM1 and its significance in diffuse large B cell lymphoma

作　　者：陈玉梅[1] 杨文秀[1] 孟青[1] 钟愉[1] 

机构地区：[1]贵阳医学院附属医院病理科,550004

出　　处：《中华血液学杂志》2011年第4期236-240,共5页Chinese Journal of Hematology

基　　金：贵州省社会发展攻关项目（2010-3054）;贵州省科学技术基金（2009-2299）

摘　　要：目的 检测含caspase募集结构的膜相关鸟氨酸激酶1（CARMA1）mRNA在弥漫大B细胞淋巴瘤（DLBCL）患者中的表达,了解其与多发性骨髓瘤癌基因蛋白（MUM1）表达的关系和对患者临床过程及预后的影响.方法 采用苏木精-伊红染色观察肿瘤的组织学特点,免疫组化染色检测肿瘤细胞的免疫表型（CD20、CD79a、CD10、MUM1、Bcl-6）,RT-PCR检测CARMA1 mRNA表达水平.收集患者的临床病理资料并随访,对所有资料进行统计学分析.结果 89例DLBCL患者中76例（85.40%）检出CARMA1 mRNA,MUM1阳性表达患者的CARMA1表达水平明显高于阴性表达者（P=0.007）,两种分子之间具有表达强度相关的趋势（P=0.084）.MUM1阳性表达患者的Ki-67阳性率明显高于阴性表达患者（P=0.030）,但两组间性别、中位年龄、临床分期、发生部位差异均无统计学意义.与CARMA1低表达组比较,高表达患者中男性多于女性（P=0.036）,其他临床病理特征两组间差异均无统计学意义.58例MUM1阳性的患者中,与CARMA1 mRNA低表达患者比较,高表达者的临床分期较早（P=0.019）,男性患者也明显多于女性患者（P=0.018）,而其他临床病理特征差异均无统计学意义.MUM1阳性和阴性表达者之间、CARMA1 mRNA高表达与低表达者之间及MUM1阳性患者的CARMA1高表达和低表达者生存状况差异均无统计学意义（P值均＞0.05）.结论 CARMA1表达与MUM1表达可能有关,男性患者表达多于女性患者.其表达可能参与了活化后B细胞样DLBCL的发生、发展过程;两者的表达对DLBCL的临床病理特征有一定影响,但与DLBCL患者的生存状况无明显相关.Objective To determine the correlation between the expression of CARMA1 mRNA and MUM1 protein, as well as its effects on clinicopathological features and prognosis of diffuse large B cell lymphoma （DLBCL）. Methods The immunophenotype （CD20, CD79a, CD10, MUM1, Bcl6） and proliferation index of DLBCL cells were examined by immunohistochemistry （IHC）. CARMA1 mRNA was detected by RT-PCR. Results CARMA1 mRNA was detected in 76 of 89 （85.40%） cases with DLBCL. The level of CARMA1 mRNA was higher in MUMl-postive group than in MUMl-negative group. No correlation was found in the expression intensity between the two molecules （P =0.084）. Ki67 positive rate was higher in MUM1 （＋） cases than in MUM1 （-） ones （P = 0. 030）. There was no difference between MUM1 （＋） and MUM1 （-） cases in sex, median age, staging, primary site and other clinicopathological features. In 58CARMA1 mRNA positive cases, low expression cases showed more in earlier stage and more males. No difference in survival status was identified between cases with and without MUM1 expression, over- and low-expression of CARMA1 mRNA ,as well as over- and low-expression of CARMA1 mRNA among 58 cases with MUM1 expression. Conclusion The expression of CARMA1 mRNA is likely associated with the expression of MUM1 and shows male predominance in DLBCL. The expression of CARMA1 may be involved with pathogenesis and progression of ABC-like DLBCL. The two molecules correlated somewhat with some clinicopathological features, but not with survival of DLBCL.

关 键 词：淋巴瘤 蛋白激酶类 CARMA1 癌基因蛋白质类 预后 

分 类 号：R733.3[医药卫生—肿瘤]