大白菜BrMYB34-3基因的3′-RACE克隆及表达  

Cloning by 3′-RACE and Expression of BrMYB34-3 Gene from Brassica rapa ssp.pekinensis

作　　者：国静[1] 刘庆霞[1] 李梦莎[1] 张耀伟[2] 阎秀峰[1] 

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心,东北油田盐碱植被恢复与重建教育部重点实验室,哈尔滨150040 [2]东北农业大学园艺学院,哈尔滨150030

出　　处：《西北植物学报》2011年第3期486-491,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中央高校基本科研业务费专项资金(DL09BA25);中国博士后科学基金资助项目(20100480963);国家自然科学基金(31070351,30670325)

摘　　要：以大白菜叶片为材料,根据NCBI数据库中Br MYB34-3基因序列的已知信息,利用3-RACE获得了Br MYB34-3基因全长,用RT-PCR方法对Br MYB34-3在不同组织中的表达进行分析.结果显示,该基因全长1 135 bp,编码280个氨基酸.Br MYB34-3具有R2R3-MYB(含有2个MYB结构域的转录因子)典型的R2、R3结构域及基序.其氨基酸序列与大白菜的Br MYB34-1和Br MYB34-2、拟南芥的At MYB34具有较高的一致性.Br MYB34-3在莲座叶、当天开的花中表达水平较高,在花序顶端表达水平较低,在根和花序轴中未检测到表达,这与拟南芥At MYB34的表达模式不同.研究表明,Br MYB34-3是大白菜中的MYB34同源基因,在功能上可能与拟南芥At MYB34基因存在差异.An R2R3-MYB gene,BrMYB34-3,was isolated from the leaves of Brassica rapa ssp.pekinensis by 3′-RACE techniques based on the partial sequence in NCBI database.The full length of BrMYB34-3 cDNA was 1 135 bp,encoding 280 amino acids.The deduced amino acid sequence contained the conserved R2 and R3 domains near the N-terminus and a conserved motif which is the characteristic of R2R3-MYB transcription factor.Phylogenetic analysis revealed that BrMYB34-3 was clustered with BrMYB34-1 and BrMYB34-2 from Brassica rapa ssp.pekinensis and AtMYB34 from Arabidopsis thaliana.Expression analysis by RT-PCR showed that BrMYB34-3 was highly expressed in rosette leaves and flowers and lowly expressed in the tip of inflorescences,but not expressed in the roots and the inflorescence stems,which is different from the expression pattern of AtMYB34 in Arabidopsis thaliana.These results indicate that BrMYB34-3 is a homologous gene of MYB34 in Brassica rapa ssp.pekinensis,and it might have at least partial different functions with Arabidopsis AtMYB34.

关 键 词：大白菜 芥子油苷 R2R3-MYB 克隆 表达分析 

分 类 号：Q789[生物学—分子生物学]