间歇性缺氧时结肠癌细胞的晚期氧化蛋白产物与血管内皮生长因子、转化生长因子β1的关系  

Relation of advanced oxidation protein products with VEGF and TGF-β_1 in colon cancer cells exposed to intermittent hypoxia

作　　者：冼乐武[1] 李涛平[1] 韦伊尔[2] 伍思培[3] 马磊[3] 

机构地区：[1]南方医科大学南方医院睡眠中心,广东广州510515 [2]广州医学院附属肿瘤医院重症医学科,广东广州510095 [3]南方医科大学肿瘤研究所,广东广州510515

出　　处：《南方医科大学学报》2011年第4期619-623,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30471720)~~

摘　　要：目的研究间歇性缺氧时结肠癌SW480细胞其晚期氧化蛋白产物(AOPP)的水平和其他氧化应激指标的关系,以及与血管内皮生长因子(VEGF)、转化生长因子β1(TGFβ1)的相关性。方法建立细胞缺氧模型,SW480细胞按缺氧条件的不同分为:间歇性缺氧组(A组)、持续性缺氧组(B组)、常氧对照组(C组)。采用ELISA方法检测AOPP和VEGF,黄嘌呤羟胺法测定超氧化物岐化酶(SOD),分光光度法测定丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX),用免疫荧光染色方法及Western blok方法测定TGFβ1的表达。结果①与对照组C组比较,A组和B组的AOPP、MDA均升高(P均<0.05);SOD、GSH-PX下降(P均<0.05)。A组与B组比较时两者的差异也具有统计学意义(P均<0.05),间歇性缺氧较持续缺氧时AOPP、MDA明显升高,SOD、GAH-PX显著下降(P均<0.05)。②间歇性缺氧时AOPP水平与MDA、VEGF、TGFβ1呈正相关(P均<0.05),与SOD为负相关关系(P均<0.05),而与GSH-PX无明显相关性。结论间歇性缺氧时SW480细胞的蛋白氧化损伤增强,较持续缺氧时明显,并且与氧化应激状态有关。缺氧时血管生成因子VEGF和TGFβ1均表达,以间歇性缺氧时显著,且其表达与AOPP水平密切相关。Objective To investigate the association of advanced oxidation protein produts（AOPP） with oxidative stress in colon cancer cells exposed to intermittent hypoxia（IH）.Methods Colon cancer SW480 cells were exposed to IH,continuous hypoxia,or normoxia.Enzyme-linked immunosorbent assay（ELISA） was employed to examine the levels of AOPP and vascular endothelial growth factor（VEGF）,xanthine oxidase assay was used to determine malonaldehyde（MDA） and glutathione peroxidase（GSH-PX）,and Western blotting and immunofluorescence assay were performed for detection of transforming growth factor-β1（TGF-β1） expression.Results Compared with the normoxia group,the two hypoxia groups showed significantly increased AOPP and MDA levels（P0.05） and lowered SOD and GSH-PX levels（P0.05）.The concentration of AOPP was positively correlated to MDA,VEGF,and TGF-β1 levels（P0.05）,but inversely to SOD.No significant correlation was found between AOPP and GSH-PX levels.Conclusion Compared with continuous hypoxia,IH results in more obvious protein oxidation in relation to oxidative stress.The increased expression of VEGF and TGF-β1 in the context of hypoxia is closely related to AOPP level.

关 键 词：间歇性缺氧 氧化应激 晚期氧化蛋白产物 血管内皮生长因子 转化生长因子Β1 

分 类 号：R735.3[医药卫生—肿瘤]