单克隆抗体MGd1对胃癌SGC-7901细胞增殖和凋亡的影响  

Effect of Monoclonal Antibody MGd1 on Proliferation and Apoptosis of Gastric Cancer Cell SGC-7901

作　　者：安艳新[1] 任贵[1] 田启飞[1] 赵青川[1] 

机构地区：[1]第四军医大学西京消化病医院肿瘤生物学国家重点实验室,陕西西安710032

出　　处：《现代生物医学进展》2011年第8期1409-1412,共4页Progress in Modern Biomedicine

基　　金：国家重点基础发展计划项目(973计划)(No.2009CB521705);国家自然科学基金(No.30971337)

摘　　要：目的:研究不同浓度的MGd1对体外培养胃癌细胞SGC-7901增殖及凋亡的影响。方法:采用MTT法测定不同浓度MGd1对SGC-7901生长抑制作用;流式细胞术(FCM)进行细胞凋亡分析。激光共聚焦显微镜观察MGd1抗原(MGd1-Ag)的亚细胞定位。结果:MTT结果显示不同浓度的MGd1均对SGC-7901细胞产生明显的抑制效应(P=0.02);流式细胞术分析发现MGd1可诱导SGC-7901发生凋亡并呈浓度和时间依赖性(P<0.01);共聚焦显微镜结果显示MGd1-Ag主要定位于细胞膜上。结论:以上结果证实胃癌特异性单抗MGd1可抑制SGC-7901的增殖并促进凋亡发生。它可能通过与细胞膜上抗原特异性结合,影响下游信号传导,从而发挥抑制效应。Objective： To investigate the effects of MGd1 with different concentration on the cell proliferation and apoptosis of gastric cancer cell line SGC-7901.Methods： Cell proliferation was detected by MTT assay and cell apoptosis was analyzed by flow cytometry.Subcellular localization of MGb2-Ag in SGC-7901 was detected by immunofluorescence staining using Laser scanning confocal microscope.Results： MGd1 could inhibit the cell proliferation and promote apoptosis of SGC7901 in dose-and time-dependent manners.The localization of MGd1-Ag was detected under confocal microscopy,and it showed that MGd1-Ag was mainly located in the membrane.Conclusion： MGd1 inhibits cell proliferation and promotes apoptosis of SGC7901 by banding with MGd1-Ag.

关 键 词：单克隆抗体 MGD1 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]