miR-15a和miR-16-1模拟物对人骨肉瘤细胞系SOSP-9607凋亡和增殖的影响  

Effects of miR-15a and miR-16-1 mimics on Apoptosis and Proliferation of Human Osteosarcoma Cell Line SOSP-9607

作　　者：蔡承魁[1] 贠喆[1] 张涛[1] 姜扩[1] 高杰[1] 裘秀春[1] 马保安[1] 

机构地区：[1]第四军医大学唐都医院全军骨科中心暨全军骨肿瘤研究所,陕西西安710038

出　　处：《现代生物医学进展》2011年第8期1417-1419,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81072194)

摘　　要：目的:探讨miR-15a和miR-16-1模拟物对于人骨肉瘤细胞系SOSP-9607凋亡和增殖的影响。方法:将SOSP-9607细胞分为实验组和对照组。实验组分为miR-15a组、miR-16-1组、miR-15a+miR-16-1组。以miR-15a组为例,采用miR-15a模拟物(hsa-miR-15a mimics)上调SOSP-9607细胞内的miR-15a表达量。对照组分为阴性对照组和空白对照组。采用流式细胞仪测定细胞凋亡率,四甲基偶氮唑蓝(MTT)法测定细胞增殖,并计算细胞增殖效率。结果:通过统计学分析,实验组凋亡率与阴性对照组凋亡率相比明显增高(P<0.05);实验组的细胞增殖率明显低于对照组(P<0.05)。结论:上调SOSP-9607细胞内miR-15a和miR-16-1的表达量可促进SOSP-9607细胞的凋亡并抑制其增殖。Objective： To investigate the effects of miR-15a and miR-16-1 mimics on apoptosis and proliferation of human osteosarcoma cell line SOSP-9607.Methods： Cultured SOSP-9607 cells were divided into experimental group and control group： the experimental groups contained miR-15a group,miR-16-1 group and miR-15a＋miR-16-1 group.Take miR-15a group for example,we use hsa-miR-15a mimics to upgrade the expression of miR-15a in SOSP-9607 cells.The control groups contained negative control and the blank control.The apoptosis were measured by flow cytometry,and MTT assay was used to examine the proliferation efficiency of human osteosarcoma cell line SOSP-9607.Results： The apoptosis ratio of experimental groups was higher than the control groups（P0.05）and the proliferation efficiency of experimental groups was higher than control groups（P0.05）.Conclusions： the miR-15a and miR-16-1 plays an important role in cell apoptosis and proliferation in SOSP-9607 cells.

关 键 词：骨肉瘤 成骨肉瘤细胞系SOSP-9607 miR-15a miR-16-1 细胞凋亡 细胞增殖 

分 类 号：R730.43[医药卫生—肿瘤] R734.2[医药卫生—临床医学]