视神经损伤后视网膜Müller细胞未折叠蛋白反应相关因子IRE-1与GLAST的表达  

Expression of Unfolded Protein Reaction Related Factor IRE-1 and GLAST in Retinal Müller Cell after Optic Nerve Injury

作　　者：马晓蕾[1] 潘峰[1] 胡丹[1] 

机构地区：[1]第四军医大学西京医院眼科全军眼科研究所,陕西西安710032

出　　处：《现代生物医学进展》2011年第8期1447-1450,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30772368)

摘　　要：目的:研究视神经损伤后视网膜Müller细胞中是否有未折叠蛋白反应(UPR)及其与L一谷氨酸/L一天门冬氨酸转运体(GLAST)的关系。方法:视神经钳夹伤模型建立成功后,运用HE染色观察视网膜神经节细胞数目改变,免疫化学染色,免疫荧光双标记,western-blot观察UPR相关因子需肌醇酶1(IRE-1)与GLAST的表达及相关性。结果:视神经钳夹伤后IRE-1与GLAST在视网膜Muller细胞上共表达,,术后第一天前呈上升趋势,在第一天达到顶峰,后呈下降趋势,第七天下降明显。结论:视神经损伤后,IRE-1与GLAST的趋势变化有一定相关性,提示未折叠蛋白反应可能是调控GLAST变化的原因之一。Objective： To investigate the unfolded protein response（UPR）in retinal Müller cells after optic nerve injury and the relationship between UPR and glutamate/aspartate transporter（GLAST）.Methods： The change of the amount of retina gangliocyte was detected by HE dying and the expression and relevance of UPR correlation factor-inositol requiring enzyme 1（IRE-1）and GLAST were detected by immunohistochemical staining,immunofluorescent double-tagging,western-blot after the modeling of trapped wound in optic nerve nipper.Results：IRE-1 and GLAST co-express in retinal Müller cells,ascend in the common trend and reach the summit one day after surgery,then drop sharply in the seventh day.Conclusions： There was relationship between the IRE-1 and GLAST in trend changes after optic nerve injured which suggests UPR may be one cause of the regulation of GLAST changes.

关 键 词：未折叠蛋白反应 MÜLLER细胞 GLAST 视网膜 大鼠 

分 类 号：R774[医药卫生—眼科]