声门上型喉癌N_0颈淋巴结微灶转移的免疫组化检测  

Immunohistochemical evaluation of micrometastases to cervical lymph nodes in supraglottic cancer

作　　者：于振坤[1] 韩德民[1] 范尔钟[1] 李颖[1] 黄志刚[1] 徐文[1] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京100730

出　　处：《中华耳鼻咽喉科杂志》1999年第5期306-308,共3页Chinese Journal of Otorhinolaryngology

基　　金：北京市科技新星计划项目资助! (编号 :953812 50 0 )

摘　　要：目的　提高声门上型喉癌颈部转移淋巴结微小转移灶的检出率。方法　应用抗细胞角蛋白 (cytokeratin,CK ,AE1/AE3)单克隆抗体 ,LSAB免疫组化法对 46例 (50侧 )常规临床病理颈部淋巴结阴性 (pN0 )的颈深上淋巴结进行监测。结果　 11例患者的 13枚淋巴结中发现微转移灶。结论　免疫组化法对微灶转移的检测比临床常规病理检查更敏感 ,因此是一临床更有使用价值的检查方法。Objective Supraglottic carcinomas of larynx tends to metastasize to cervical lymph nodes The regular pathological method of diagnosis is easy to have the micrometastasis missed, and serial sectioning is too difficult be used in the regular pathological examinations The aim of this study was to evaluate the value of immunohistochemical assay in the detection of micrometastasis Methods Monoclonal antibodies against cytokeratin (AE1/AE3) were used to detect the cervical lymph nodes of 46 cases (50 sides of neck) of supraglottic carcinoma with pathologically negative findings (pN 0) Results Micrometastases were identified in 13 lymph nodes of 11 cases Conclusion It is suggested that the detection of lymph node micrometastases with immunohistochemical method is more sensitive than that of regular pathologic method It is comparable to serial sectioning Therefore, It is a valuable clinical method to detect the early lymph node metastasis

关 键 词：喉肿瘤 淋巴转移 角蛋白 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]