中国沿海养殖牡蛎尼氏单孢子虫感染情况调查  

Investigation of Haplosporidium nelsoni Infection in Breeding Oyster of China Bays

作　　者：王宗祥[1,2] 吴绍强[2] 杨晓野[1] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]中国检验检疫科学研究院,北京100029

出　　处：《中国畜牧兽医》2011年第5期158-160,共3页China Animal Husbandry & Veterinary Medicine

基　　金：质检公益专项(201010016);国家质检总局科研基金(2007IK038)资助

摘　　要：单孢子虫病是严重危害世界贝类养殖业的主要疫病之一。本研究选取中国沿海养殖的牡蛎作为研究对象,采用OIE推荐的PCR方法结合测序技术,对采自中国沿海4个监测点养殖牡蛎的感染情况进行调查,结果表明,这些监测点养殖的牡蛎均不同程度感染了单孢子虫。经PCR测序及序列分析表明感染种类均为尼尔森单孢子虫(Haplosporidium nelsoni)。Haplosporidiosis is a serious mollusc disease caused by parasitic Haplosporidium. The Haplosporidium-specific PCR detecting method, recommanded by World Animal Health Organization （OIE）, was adopted to investigate Haplosporidiosis prevalence of oyster in three sampling sites of China east bays. Results showed that there was Haplosporidium prevalence in all the four sites in different degree. Through combining with DNA sequencing and sequence analysis, the infection species was identified to he Haplosporidium nelsoni.

关 键 词：单孢子虫 PCR 测序 感染情况调查 序列分析 

分 类 号：S852.723[农业科学—基础兽医学]